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公开(公告)号：US09803165B2
公开(公告)日：2017-10-31
申请号：US13610954
申请日：2012-09-12
申请人： Wilson Moya , Alison Dupont
发明人： Wilson Moya , Alison Dupont
摘要： Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.
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公开(公告)号：US20130203969A1
公开(公告)日：2013-08-08
申请号：US13566320
申请日：2012-08-03
申请人： Jad Jaber , Wilson Moya , Ajish Potty , Alison Dupont , Matthew T. Stone , Mikhail Kozlov
发明人： Jad Jaber , Wilson Moya , Ajish Potty , Alison Dupont , Matthew T. Stone , Mikhail Kozlov
IPC分类号： C07K16/00
CPC分类号： C07K16/00 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , C07K1/30
摘要： The present invention relates to novel and improved methods for the purification of biomolecules. In particular, the present invention relates to methods of protein purification which employ small molecules, which include at least one non-polar group and at least one cationic group or which include at least one non-polar group and at least one anionic group.
摘要翻译： 本发明涉及用于纯化生物分子的新颖和改进的方法。 特别地，本发明涉及使用包括至少一个非极性基团和至少一个阳离子基团或包括至少一个非极性基团和至少一个阴离子基团的小分子的蛋白质纯化方法。
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公开(公告)号：US20100190963A1
公开(公告)日：2010-07-29
申请号：US12633141
申请日：2009-12-08
申请人： Wilson Moya , Alison Dupont
发明人： Wilson Moya , Alison Dupont
摘要： Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.
摘要翻译： 用于样品制备或加工的容器，例如生物质培养或加工，以及任选的样品纯化。 在某些实施方案中，反应器是包括搅动的细胞装置的生物反应器，其模拟切向流过滤器以减少或消除由所产生的固体引起的堵塞。 在某些实施方案中，固体包含沉淀物或絮状物或珠粒，例如包含结合感兴趣的生物分子的聚合物和杂质的固体颗粒。 在其方法方面，本文公开的实施方案包括纯化和分离衍生自细胞培养液的生物分子。 所述方法包括在容器中进行样品制备或加工，培养生物质; 通过从培养的肉汤中沉淀或絮凝目的生物分子产生固体; 防止固体通过搅拌沉降在容器中; 和纯化，例如通过洗脱感兴趣的生物分子并过滤它们。
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公开(公告)号：US20130005950A1
公开(公告)日：2013-01-03
申请号：US13610954
申请日：2012-09-12
申请人： Wilson Moya , Alison Dupont
发明人： Wilson Moya , Alison Dupont
IPC分类号： C07K1/36
摘要： Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.
摘要翻译： 用于样品制备或加工的容器，例如生物质培养或加工，以及任选的样品纯化。 在某些实施方案中，反应器是包括搅动的细胞装置的生物反应器，其模拟切向流过滤器以减少或消除由所产生的固体引起的堵塞。 在某些实施方案中，固体包含沉淀物或絮状物或珠粒，例如包含结合感兴趣的生物分子的聚合物和杂质的固体颗粒。 在其方法方面，本文公开的实施方案包括纯化和分离衍生自细胞培养液的生物分子。 所述方法包括在容器中进行样品制备或加工，培养生物质; 通过从培养的肉汤中沉淀或絮凝目的生物分子产生固体; 防止固体通过搅拌沉降在容器中; 和纯化，例如通过洗脱感兴趣的生物分子并过滤它们。
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