公开(公告)号：US5296366A

公开(公告)日：1994-03-22

申请号：US703778

申请日：1991-05-22

申请人： Mario P. E. Garcia ,  Aimee P. Felipe ,  Roger R. Chaplen ,  Ricardo S. Doce ,  Luciano F. H. Marrero ,  Pedro R. Collazo ,  Anaisel C. Ramirez ,  Emilio A. M. Munoz ,  Walfrido B. Martinez ,  Magalys C. Somavilla ,  Alicia P. Fernandez ,  Jose d. d. Garcia ,  Luis S. H. Martinez

发明人： Mario P. E. Garcia ,  Aimee P. Felipe ,  Roger R. Chaplen ,  Ricardo S. Doce ,  Luciano F. H. Marrero ,  Pedro R. Collazo ,  Anaisel C. Ramirez ,  Emilio A. M. Munoz ,  Walfrido B. Martinez ,  Magalys C. Somavilla ,  Alicia P. Fernandez ,  Jose d. d. Garcia ,  Luis S. H. Martinez

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61P7/02 ,  C07K14/315 ,  C12N1/19 ,  C12N1/21 ,  C12N9/70 ,  C12N15/52 ,  C12N15/58 ,  C12N15/81 ,  C12R1/19 ,  C12R1/465 ,  C12R1/84 ,  C12N15/70

CPC分类号： C07K14/3153 ,  C12N15/815 ,  A61K38/00

摘要： The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms.The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which it includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase.The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.

摘要翻译： 本发明涉及生物技术和遗传工程领域，特别涉及编码链激酶的新型核苷酸序列，以及由其获得的用于转化各种宿主生物的重组DNA。 本发明基于分离编码来自C型（菌株ATCC-9542）的等位链球菌的链激酶的新基因及其在原核（大肠杆菌）和真核（巴斯德毕赤酵母）宿主中的克隆和表达，用于 其包括含有所述基因的遗传序列的表达载体，以及用能够产生链激酶的这些载体转化的微生物。 由此获得的蛋白质可用于临床医学中作为治疗剂，用于治疗诸如血栓栓塞障碍（包括冠状动脉血栓形成）的疾病。