公开(公告)号：US07880150B2

公开(公告)日：2011-02-01

申请号：US12128948

申请日：2008-05-29

申请人： Stefan Hell ,  Christian Eggeling ,  Alexander Egner ,  Jonas Fölling ,  Andreas Schönle ,  Mariano Bossi

发明人： Stefan Hell ,  Christian Eggeling ,  Alexander Egner ,  Jonas Fölling ,  Andreas Schönle ,  Mariano Bossi

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e. worse) than an average spacing between closest neighboring molecules of the substance in the specimen; the specimen is exposed to light in a region which has dimensions larger than the spatial resolution limit, fractions of the substance alternately being excited by the light to emit fluorescent light and converted into their second state, and at least 10% of the molecules of the substance that are respectively in the first state lying at a distance from their closest neighboring molecules in the first state which is greater than the spatial resolution limit; and the fluorescent light, which is spontaneously emitted by the substance from the region, is registered in a plurality of images recorded by the sensor array during continued exposure of the specimen to the light.

摘要翻译： 对于样本中感兴趣的结构的高空间分辨率成像，物质选自具有荧光第一状态和非荧光第二状态的物质组; 其可以通过将它们激发成荧光的光从其第一状态分数转换成第二状态，并且从它们的第二状态返回到其第一状态; 将感兴趣的标本的结构成像到传感器阵列上，成像的空间分辨率极限比样品中物质的最接近的相邻分子之间的平均间距更大（即更差） 试样在具有大于空间分辨率极限的尺寸的区域中暴露于光，该物质的部分交替地被光激发以发射荧光并转换成其第二状态，并且至少10％的 分别处于第一状态的物质，其处于与第一状态相比最大相邻分子一定距离，该距离大于空间分辨率极限; 并且由该区域内的物质自发地发出的荧光被记录在传感器阵列记录的多个图像中，该样本在样品持续暴露于光下。

公开(公告)号：US20110160083A1

公开(公告)日：2011-06-30

申请号：US13013359

申请日：2011-01-25

申请人： Stefan Hell ,  Christian Eggeling ,  Alexander Egner ,  Jonas Fölling ,  Andreas Schönle ,  Mariano Bossi

发明人： Stefan Hell ,  Christian Eggeling ,  Alexander Egner ,  Jonas Fölling ,  Andreas Schönle ,  Mariano Bossi

IPC分类号： C40B30/04 ,  C40B30/10

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.

摘要翻译： 对于结构的成像，结构用可以通过开关信号从第一状态转换为第二状态并且仅在其状态之一提供光学测量信号的物质标记。 施加开关信号，使得处于测量信号提供状态的物质的至少10％的分子距离它们最近的邻近物一个距离，其大于将样本成像到传感器阵列上的空间分辨率极限， 其又大于物质分子之间的平均距离。 根据记录有传感器阵列的测量信号的强度分布，该位置仅对于在测量信号提供状态中离它们最近的相邻分子一定距离的物质的那些分子确定，其大于空间分辨率极限 。

公开(公告)号：US20110081653A1

公开(公告)日：2011-04-07

申请号：US12949159

申请日：2010-11-18

申请人： Stefan W. HELL ,  Jonas FÖLLING ,  Christian EGGELING ,  Alexander EGNER ,  Andreas SCHÖNLE ,  Mariano BOSSI

发明人： Stefan W. HELL ,  Jonas FÖLLING ,  Christian EGGELING ,  Alexander EGNER ,  Andreas SCHÖNLE ,  Mariano BOSSI

IPC分类号： G01N21/64 ,  G21K5/00 ,  C12Q1/68

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： In high spatial resolution imaging, a structure in a specimen is marked with a substance which, in a first electronic state, is excited by light of one wavelength to emit fluorescent light, which is also converted from its first into a second electronic state by that light, and which returns from its second into its first electronic state. The specimen is imaged onto a sensor at a spatial resolution not resolving an average spacing between neighboring molecules of the substance, and exposed to the light at such an intensity that the molecules in the first state are alternately excited to emit fluorescent light and converted into their second state, and that at least 10% of the molecules presently in their first state lie at a distance from their closest neighboring molecules in their first state which is greater than the spatial resolution of the imaging onto the sensor.

摘要翻译： 在高空间分辨率成像中，样本中的结构用物质标记，该物质在第一电子状态下被一个波长的光激发以发射荧光，其也从其第一个电子状态转换成第二电子状态 光，从第二个回到第一个电子状态。 将样本以不分辨物质的相邻分子之间的平均间隔的空间分辨率成像到传感器上，并以如此强度暴露于光，使得第一状态下的分子被交替地激发以发射荧光并转换成它们 并且目前处于其第一状态的至少10％的分子处于与它们的第一状态中最接近的相邻分子相距一定距离，其大于成像在传感器上的空间分辨率。

公开(公告)号：US20100140506A1

公开(公告)日：2010-06-10

申请号：US12687969

申请日：2010-01-15

申请人： Christian EGGELING ,  Andreas SCHÖNLE ,  Stefan W. HELL ,  Michael HILBERT

发明人： Christian EGGELING ,  Andreas SCHÖNLE ,  Stefan W. HELL ,  Michael HILBERT

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  G01N21/6408 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6439

摘要： A method of determining a measurement value on the basis of a plurality of single molecule events of marker molecules in a sample comprises the steps of selecting the marker molecules from a group of marker molecules which are transferable between a measurable state in which a measurement signal necessary for determining the at least one measurement value is obtainable from the marker molecules and a non-measurable state, of providing the marker molecules in the sample at such an absolute concentration that the at least one measurement value is not determinable, if all marker molecules are in their measurable state, and adjusting a measurement concentration of the marker molecules in the measurable state by means of applying the physical signal to the sample at such an intensity that the at least one measurement value is determinable within a defined measurement area of the sample.

摘要翻译： 基于样品中标记分子的多个单分子事件确定测量值的方法包括以下步骤：从一组标记分子中选择标记分子，所述标记分子可在可测量状态之间转移，其中测量信号必需 用于确定所述至少一个测量值可从所述标记分子和不可测量的状态获得，以如下绝对浓度提供所述标记分子，所述绝对浓度至少一个测量值不能确定，如果所有标记分子是 在可测量的状态下，并且以可测量状态的标记分子的测量浓度通过将样品的物理信号以样品的限定测量区域内的至少一个测量值确定的强度来调节。

公开(公告)号：US08174692B2

公开(公告)日：2012-05-08

申请号：US12949159

申请日：2010-11-18

申请人： Stefan W. Hell ,  Jonas Fölling ,  Christian Eggeling ,  Alexander Egner ,  Andreas Schönle ,  Mariano Bossi

发明人： Stefan W. Hell ,  Jonas Fölling ,  Christian Eggeling ,  Alexander Egner ,  Andreas Schönle ,  Mariano Bossi

IPC分类号： G01J3/30

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： In high spatial resolution imaging, a structure in a specimen is marked with a substance which, in a first electronic state, is excited by light of one wavelength to emit fluorescent light, which is also converted from its first into a second electronic state by that light, and which returns from its second into its first electronic state. The specimen is imaged onto a sensor at a spatial resolution not resolving an average spacing between neighboring molecules of the substance, and exposed to the light at such an intensity that the molecules in the first state are alternately excited to emit fluorescent light and converted into their second state, and that at least 10% of the molecules presently in their first state lie at a distance from their closest neighboring molecules in their first state which is greater than the spatial resolution of the imaging onto the sensor.

摘要翻译： 在高空间分辨率成像中，样本中的结构用物质标记，该物质在第一电子状态下被一个波长的光激发以发射荧光，其也从其第一个电子状态转换成第二电子状态 光，从第二个回到第一个电子状态。 将样本以不分辨物质的相邻分子之间的平均间隔的空间分辨率成像到传感器上，并以如此强度暴露于光，使得第一状态下的分子被交替地激发以发射荧光并转换成它们 并且目前处于其第一状态的至少10％的分子处于与它们的第一状态中最接近的相邻分子相距一定距离，其大于成像在传感器上的空间分辨率。

公开(公告)号：US08084754B2

公开(公告)日：2011-12-27

申请号：US13013359

申请日：2011-01-25

申请人： Stefan Hell ,  Christian Eggeling ,  Alexander Egner ,  Jonas Fölling ,  Andreas Schönle ,  Mariano Bossi

发明人： Stefan Hell ,  Christian Eggeling ,  Alexander Egner ,  Jonas Fölling ,  Andreas Schönle ,  Mariano Bossi

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.

摘要翻译： 对于结构的成像，结构用可以通过开关信号从第一状态转换为第二状态并且仅在其状态之一提供光学测量信号的物质标记。 施加开关信号，使得处于测量信号提供状态的物质的至少10％的分子距离它们最近的邻近物一个距离，其大于将样本成像到传感器阵列上的空间分辨率极限， 其又大于物质分子之间的平均距离。 根据记录有传感器阵列的测量信号的强度分布，该位置仅对于在测量信号提供状态中离它们最近的相邻分子一定距离的物质的那些分子确定，其大于空间分辨率极限 。