公开(公告)号：US08004676B1

公开(公告)日：2011-08-23

申请号：US12069700

申请日：2008-02-11

申请人： Paras N. Prasad ,  Przemyslaw P. Markowicz ,  Wing Cheung Law ,  Andrei Kabashin ,  Sergiy Patskovsky

发明人： Paras N. Prasad ,  Przemyslaw P. Markowicz ,  Wing Cheung Law ,  Andrei Kabashin ,  Sergiy Patskovsky

IPC分类号： G01J4/00

CPC分类号： G01N21/553 ,  G01N2021/212

摘要： A method is provided for detection of analytes using the Surface Plasmon Resonance effect. The method comprises providing a metal film on a transparent substrate. The free surface of the metal film is exposed to a test sample. An anlyte in the sample can interact directly with the metal film or via analyte binding molecules (ABMs) complexed to the film. Light is directed incident to the surface of film in contact with the substrate. Light is reflected from the surface of the film under SPR conditions. The reflected light is collected and the second and/or third harmonics of the resulting electrical signal, which are indicative of the phase and polarization state of the reflected light, are determined. The second and third harmonics are correlated to the presence and/or concentration of the analyte.

摘要翻译： 提供了一种使用表面等离子体共振效应检测分析物的方法。 该方法包括在透明基底上提供金属膜。 将金属膜的自由表面暴露于测试样品。 样品中的非离子液体可以直接与金属膜或通过与膜复合的分析物结合分子（ABM）相互作用。 光引导入射到与基板接触的膜的表面。 在SPR条件下，光从膜的表面反射。 收集反射光，并且确定表示反射光的相位和极化状态的所得电信号的第二和/或第三谐波。 第二和第三谐波与分析物的存在和/或浓度相关。