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    Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector
    1.
    发明授权
    Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector 失效
    通过托博韦病毒载体在转染植物中细胞质抑制基因表达

    公开(公告)号：US06700040B2

    公开(公告)日：2004-03-02

    申请号：US09771035

    申请日：2001-01-25

    申请人： Peter D. Roberts ,  Andy A. Vaewhongs ,  Monto H. Kumagai

    发明人： Peter D. Roberts ,  Andy A. Vaewhongs ,  Monto H. Kumagai

    IPC分类号： C12N1583

    CPC分类号： A01H1/04 ,  A01H5/12 ,  C12N15/1034 ,  C12N15/8261 ,  C12Q1/68 ,  Y02A40/146

    摘要： This invention is directed to a monopartite RNA viral vector comprising modified tobravirus RNA-1 comprising an inserted foreign RNA sequence. This invention is also directed to a bipartite RNA viral vector derived from a tobravirus, wherein the vector comprises one or more foreign RNA sequences. The invention is directed to a method of silencing one or more endogenous plant host genes and a method of simultaneously silencing a plant host gene and expressing a foreign gene in a plant host. Such methods comprise infecting a plant host with a bipartite vector comprising modified tobravirus RNA-1 and RNA-2.

    摘要翻译： 本发明涉及包含经插入的外源RNA序列的修饰的载体RNA-1的单分子RNA病毒载体。 本发明还涉及衍生自托巴威病毒的二分之一RNA病毒载体，其中所述载体包含一个或多个外源RNA序列。 本发明涉及沉默一种或多种内源性植物宿主基因的方法和同时使植物宿主基因沉默并在植物宿主中表达外源基因的方法。 这样的方法包括用包含修饰的tobravirus RNA-1和RNA-2的二重载体感染植物宿主。