公开(公告)号：US5989897A

公开(公告)日：1999-11-23

申请号：US47026

申请日：1998-03-24

申请人： Lorraine Pillus ,  Astrid Clarke ,  Joanna Lowell ,  Sandra Jacobson ,  Cheryl Reifsnyder

发明人： Lorraine Pillus ,  Astrid Clarke ,  Joanna Lowell ,  Sandra Jacobson ,  Cheryl Reifsnyder

IPC分类号： C07K14/395 ,  C12N1/19 ,  C12N15/81 ,  C12N1/18 ,  C12N15/31 ,  C12N15/63

CPC分类号： C07K14/395 ,  C12N15/81

摘要： The present invention provides the yeast genes SAS2, SAS3 and ESA1 and the proteins encoded thereby. SAS2, SAS3 and ESA1 genes of members of the genus Saccharomyces are provided, particularly the SAS2, SAS3 and ESA1 genes of S. cerevisiae. Also provided are yeast SAS2, SAS3 and ESA1 coding sequences. Specifically provided are the SAS2, SAS3 and ESA1 coding sequences of members of the genus Saccharomyces, and more specifically of S. cerevisiae. Genes of this invention comprise protein coding sequences as well as the regulatory regions that control expression of the encoded protein. Of most interest are SAS2, SAS3, and ESA1 genes of yeast including those of the genus Saccharomyces which are 90% or more homologous to the corresponding genes of S. cerevisiae. Specifically provided are DNA constructs comprising purified and isolated DNA molecules comprising SAS2, SAS3 or ESA1 coding sequences that encode proteins from a strain of S. cerevisiae.

摘要翻译： 本发明提供了酵母基因SAS2，SAS3和ESA1以及由此编码的蛋白质。 提供了Saccharomyces属的SAS2，SAS3和ESA1基因，特别是酿酒酵母的SAS2，SAS3和ESA1基因。 还提供了酵母SAS2，SAS3和ESA1编码序列。 具体提供的是Saccharomyces属的成员的SAS2，SAS3和ESA1编码序列，更特别的是酿酒酵母。 本发明的基因包括蛋白质编码序列以及控制编码蛋白质表达的调控区域。 最感兴趣的是酵母的SAS2，SAS3和ESA1基因，包括与酿酒酵母的相应基因90％或更多同源的酵母属的那些。 特别提供了包含纯化和分离的DNA分子的DNA构建体，其包含编码来自酿酒酵母菌株的蛋白质的SAS2，SAS3或ESA1编码序列。