公开(公告)号：US20240174722A1

公开(公告)日：2024-05-30

申请号：US18281117

申请日：2022-03-11

Applicant: TENZA, INC.

Inventor： Anik Debnath ,  Ameet Shetty

IPC: C07K14/395 ,  A61K36/064 ,  A61K38/00 ,  C12N1/18 ,  C12R1/685 ,  C12R1/84 ,  C12R1/87 ,  C12R1/885

CPC classification number: C07K14/395 ,  A61K36/064 ,  C12N1/185 ,  A61K38/00 ,  C07K2319/02 ,  C12R2001/685 ,  C12R2001/84 ,  C12R2001/87 ,  C12R2001/885

Abstract: Provided herein are signal peptides that direct secretion of expressed payload proteins in yeast. Methods of using the signal peptides for therapeutic and non-therapeutic utilities are also provided. Compositions comprising yeast comprising the signal peptides and methods of using said yeast comprising the signal peptides for therapeutic and non-therapeutic utilities are also provided. Methods to design and generate the disclosed signal peptides are also provided.

公开(公告)号：US11980640B2

公开(公告)日：2024-05-14

申请号：US16613187

申请日：2018-05-15

Applicant: The United States of America,as represented by the Secretary,Department of Health and Human Services

Inventor： Haiying Qin ,  Crystal L Mackall ,  Terry J. Fry

IPC: A61K35/17 ,  A61P35/00 ,  C07K14/395 ,  C07K14/725 ,  C07K16/28 ,  C12N15/85

CPC classification number: A61K35/17 ,  A61P35/00 ,  C07K14/395 ,  C07K14/7051 ,  C07K16/2803 ,  C12N15/85

Abstract: An embodiment of the invention provides bicistronic chimeric antigen receptor (CAR) amino acid constructs. Nucleic acids, recombinant expression vectors, host cells, populations of cells, and pharmaceutical compositions relating to the CAR constructs are disclosed. Methods of detecting the presence of cancer in a mammal and methods of treating or preventing cancer in a mammal are also disclosed. Methods of making the CAR constructs are disclosed.

公开(公告)号：US20240150416A1

公开(公告)日：2024-05-09

申请号：US18341669

申请日：2023-06-26

Applicant: BOLT THREADS, INC.

Inventor： Joshua Kittleson ,  Thomas Stevens ,  Rena Hill ,  Carlos Gustavo Pesce ,  David N. Breslauer ,  Daniel M. Widmaier

IPC: C07K14/435 ,  C07K14/395 ,  C12P21/02

CPC classification number: C07K14/43586 ,  C07K14/395 ,  C12P21/02 ,  C07K2319/02

Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the α-mating factor (αMF) of Saccharomyces cerevisiae and a non-αMF signal peptide.

公开(公告)号：US20240084243A1

公开(公告)日：2024-03-14

申请号：US18344790

申请日：2023-06-29

Applicant: Clara Foods Co.

Inventor： Weixi ZHONG ,  Logan Hurst

IPC: C12N1/16 ,  C07K14/395 ,  C12N9/24 ,  C12N15/81

CPC classification number: C12N1/16 ,  C07K14/395 ,  C12N9/2402 ,  C12N15/815 ,  C12R2001/84

Abstract: The present disclosure provides engineered eukaryotic cells comprising a surface displayed fusion proteins comprising a catalytic domain of an enzyme and an anchoring domain of a glycosylphosphatidylinositol (GPI)-anchored protein and methods of use.

公开(公告)号：US20240026325A1

公开(公告)日：2024-01-25

申请号：US18346095

申请日：2023-06-30

Applicant: Clara Foods Co.

Inventor： Weixi ZHONG

IPC: C12N9/24 ,  C07K14/395 ,  C07K14/435 ,  C12N1/16

CPC classification number: C12N9/2402 ,  C07K14/395 ,  C07K14/435 ,  C12N1/16 ,  C12Y302/01096 ,  C12R2001/84

Abstract: The present disclosure provides engineered eukaryotic cells comprising a surface displayed catalytic domain of an endoglycosidase and methods of use.

公开(公告)号：US20230355728A1

公开(公告)日：2023-11-09

申请号：US18056554

申请日：2022-11-17

Applicant: THE SCRIPPS RESEARCH INSTITUTE

Inventor： Travis Young ,  Chanhyuk Kim ,  Peter G. Schultz

IPC: C12N5/0783 ,  C07K14/395 ,  A61K39/395 ,  C07K16/28 ,  A61K39/00

CPC classification number: A61K39/0002 ,  A61K39/3955 ,  C07K14/395 ,  C07K16/2803 ,  C07K16/2887 ,  C12N5/0636 ,  A61K38/00

Abstract: Disclosed herein are chimeric antigen receptor effector cells (CAR-ECs) and CAR-EC switches. The switchable CAR-ECs are generally T cells. The one or more chimeric antigen receptors may recognize a peptidic antigen on the CAR-EC switch. The CAR-ECs and switches may be used for the treatment of a condition in a subject in need thereof.

公开(公告)号：US11725213B2

公开(公告)日：2023-08-15

申请号：US17341224

申请日：2021-06-07

Applicant: Wisconsin Alumni Research Foundation

Inventor： Chris Todd Hittinger ,  EmilyClare Patricia Baker

IPC: C12N1/14 ,  C12N15/00 ,  C12P7/00 ,  C12N15/81 ,  C12N1/16 ,  C07K14/395

CPC classification number: C12N15/81 ,  C12N1/16 ,  C12P7/00 ,  C07K14/395

Abstract: Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.

公开(公告)号：US20180312878A1

公开(公告)日：2018-11-01

申请号：US15754524

申请日：2016-09-02

Applicant: VIB VZW ,  KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D

Inventor： Kevin Verstrepen ,  Yudi Yang ,  Veerle Saels ,  Karin Voordeckers ,  Beatriz Herrera

IPC: C12P7/06 ,  C07K14/395 ,  C12N15/81

CPC classification number: C07K14/395 ,  C12P7/06 ,  Y02E50/17

Abstract: The present application relates to the field of yeast and, specifically, to the identification of yeast alleles that are involved in maximal alcohol accumulation and/or in tolerance to high alcohol levels. The alcohol may be, e.g., ethanol. The identified alleles can be combined or stacked with each other to construe and/or select high alcohol tolerant yeasts, most notably Saccharomyces species.

公开(公告)号：US20180305700A1

公开(公告)日：2018-10-25

申请号：US15767901

申请日：2016-10-13

Applicant: The Research Foundation for the State University of New York

Inventor： Stewart N. LOH ,  Jeung-Hoi HA ,  Adam R. BLANDEN ,  Alan BLAYNEY

IPC: C12N15/62 ,  C12N15/65 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C07K19/00

CPC classification number: C12N15/62 ,  C07K14/245 ,  C07K14/395 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/10 ,  C07K2319/50 ,  C12N15/65 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12N2310/3517 ,  C12N2310/3519 ,  C12N2510/02 ,  C12N2810/50

Abstract: Provided are compositions and methods for enhancing recombinant protein production. The compositions and methods involve use of Ribose Binding Protein (RBP) as a segment of a fusion polypeptide, whereby the RBP segment enhances production of the fusion protein. The fusion proteins contain the RBP sequentially in a single fusion protein with a polypeptide for which enhanced expression is desired. Recombinant expression vectors encoding the fusion proteins that contain and RBP segment are included, as are cells that contain the expression vectors. Methods for separating fusion proteins and for liberating a polypeptide segment that is part of the fusion protein are also provided.

公开(公告)号：US20180235263A1

公开(公告)日：2018-08-23

申请号：US15751674

申请日：2016-08-15

Applicant: DSM IP ASSETS B.V.

Inventor： Viktor Marius BOER ,  Priscilla ZWARTJENS ,  Eric VAN DEN BERG

IPC: A23L27/30 ,  A23L2/60 ,  C12N9/24 ,  C12N1/16 ,  C12P19/56 ,  C12P15/00 ,  C07K14/395 ,  C07K14/40

CPC classification number: A23L27/36 ,  A23L2/60 ,  C07K14/39 ,  C07K14/395 ,  C07K14/40 ,  C12N1/16 ,  C12N9/2402 ,  C12P15/00 ,  C12P19/56

Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.