公开(公告)号：US20160375439A1

公开(公告)日：2016-12-29

申请号：US15130930

申请日：2016-04-16

申请人： Paul Chi Hang Li ,  Avid Khamenehfar

发明人： Paul Chi Hang Li ,  Avid Khamenehfar

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N33/50

CPC分类号： B01L3/502753 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2400/0424 ,  G01N15/1484 ,  G01N33/5011 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493

摘要： Particles, such as cells, are isolated for conducting single-particle measurement. Isolation of rare cells, such as circulating tumor cells (CTCs), from blood is technically challenging because they are small in numbers. An integrated microfluidic biochip, dubbed as CTC chip, was designed and fabricated for conducting tumor cell isolation. As CTCs are usually multidrug resistance (MDR), the effect of MDR inhibitors on chemotherapeutic drug accumulation in the isolated single tumor cell is measured. In this invention, label-free isolation of the rare tumor cells was conducted based on cell size difference. The major advantages of the CTC chip are the ability of fast cell isolation, followed by multiple rounds of single-cell measurement, suggesting a potential assay for detecting the drug responses based on the liquid biopsy of cancer patients.

摘要翻译： 隔离颗粒，如细胞，进行单粒子测量。 从血液中分离罕见细胞，如循环肿瘤细胞（CTC），在技术上是具有挑战性的，因为它们的数量很少。 被设计和制造用于进行肿瘤细胞分离的集成微流体生物芯片，被称为CTC芯片。 由于CTC通常是多药耐药性（MDR），测量MDR抑制剂对分离的单个肿瘤细胞中化疗药物积累的影响。 在本发明中，基于细胞大小差异进行稀有肿瘤细胞的无标记分离。 CTC芯片的主要优点是快速细胞分离的能力，随后进行多轮单细胞测量，表明基于癌症患者的液体活检检测药物反应的潜在测定。