公开(公告)号：US20110112813A1

公开(公告)日：2011-05-12

申请号：US12995064

申请日：2009-05-29

申请人： Fumiaki Abe ,  Ayako Uchijima ,  Ayako Horigome

发明人： Fumiaki Abe ,  Ayako Uchijima ,  Ayako Horigome

IPC分类号： G06F17/11

CPC分类号： G06F19/00 ,  C12Q1/06

摘要： The present invention provides a method for estimating survival cell count, which is able to calculate an accurately estimated result of the survival cell count of a specific strain in a probiotic product with respect to its storage period, and to shorten the time for developing the product, and a method for setting a guaranteed cell count, which is able to readily calculate a guaranteed cell count of a specific strain in a probiotic product within its quality guarantee period. The method for estimating the survival cell count is characterized by estimating the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10 nt=Log10 n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)×1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.

摘要翻译： 本发明提供一种用于估计存活细胞计数的方法，其能够计算益生菌产品相对于其储存期的特定菌株的存活细胞计数的准确估计结果，并缩短开发产品的时间 以及用于设定保证细胞计数的方法，其能够容易地计算其质量保证期内益生菌产品中特定菌株的保证细胞计数。 用于估计存活细胞计数的方法的特征在于根据以下等式（I）估计储存后组合物中包含的特定菌株的存活细胞计数nt（CFU / g）。 （I）Log10 nt = Log10 n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表保存期（天）×1/30，nt表示 储存期t（天）后组合物中所含的菌株的存活细胞计数（CFU / g），n0表示储存开始时组合物中包含的菌株的活菌数（CFU / g），T 代表储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的菌株特异性系数，BT和DT代表对菌株特异性的实验测定常数。

公开(公告)号：US08420128B2

公开(公告)日：2013-04-16

申请号：US12867979

申请日：2009-03-31

申请人： Ayako Horigome ,  Mai Murata ,  Koji Yamauchi ,  Mitsunori Takase ,  Yasuhiro Takeda ,  Junichi Hashimoto ,  Ikumi Kojima

发明人： Ayako Horigome ,  Mai Murata ,  Koji Yamauchi ,  Mitsunori Takase ,  Yasuhiro Takeda ,  Junichi Hashimoto ,  Ikumi Kojima

IPC分类号： A01N59/20 ,  A61K33/34

CPC分类号： A61K47/42 ,  A23L33/19 ,  A61K9/0019 ,  A61K9/0095 ,  A61K9/08 ,  A61K9/2063 ,  A61K9/4866 ,  A61K38/40 ,  A61K47/26

摘要： An agent for thermally stabilizing lactoferrin, which comprises a nucleic acid as an active ingredient, can be added to lactoferrin to impart thermal stability to lactoferrin. Thermally stabilized lactoferrin can be heat-sterilized at a pH value around a neutral pH value while keeping its activity.

摘要翻译： 可以将含有核酸作为活性成分的用于热稳定的乳铁蛋白的试剂加入到乳铁蛋白中以赋予乳铁蛋白热稳定性。 热稳定的乳铁蛋白可以在pH值约为中性pH值的同时保持其活性而被热灭菌。

公开(公告)号：US08700373B2

公开(公告)日：2014-04-15

申请号：US12995064

申请日：2009-05-29

申请人： Fumiaki Abe ,  Ayako Uchijima ,  Ayako Horigome

发明人： Fumiaki Abe ,  Ayako Uchijima ,  Ayako Horigome

IPC分类号： G06G7/58 ,  G06F19/00

CPC分类号： G06F19/00 ,  C12Q1/06

摘要： The present invention provides a method for estimating survival cell count, which is able to calculate an accurately estimated result of the survival cell count of a specific strain in a probiotic product with respect to its storage period, and to shorten the time for developing the product, and a method for setting a guaranteed cell count, which is able to readily calculate a guaranteed cell count of a specific strain in a probiotic product within its quality guarantee period. The method for estimating the survival cell count is characterized by estimating the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10 nt=Log10 n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)×1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.

摘要翻译： 本发明提供一种用于估计存活细胞计数的方法，其能够计算益生菌产品相对于其储存期的特定菌株的存活细胞计数的准确估计结果，并缩短开发产品的时间 以及用于设定保证细胞计数的方法，其能够容易地计算其质量保证期内益生菌产品中特定菌株的保证细胞计数。 用于估计存活细胞计数的方法的特征在于根据以下等式（I）估计储存后组合物中包含的特定菌株的存活细胞计数nt（CFU / g）。 （I）Log10 nt = Log10 n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表保存期（天）×1/30，nt表示 储存期t（天）后组合物中所含菌株的存活细胞计数（CFU / g），n0表示储存开始时组合物中包含的菌株的活菌数（CFU / g），T 代表储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的菌株特异性系数，BT和DT代表对菌株特异性的实验测定常数。

公开(公告)号：US20110071768A1

公开(公告)日：2011-03-24

申请号：US12995066

申请日：2009-05-29

申请人： Fumiaki Abe ,  Ayako Uchijima ,  Masamichi Muto ,  Ayako Horigome

发明人： Fumiaki Abe ,  Ayako Uchijima ,  Masamichi Muto ,  Ayako Horigome

IPC分类号： G06F19/00 ,  G01N33/48

CPC分类号： C12Q1/06

摘要： By accurately estimating the survival cell count in a probiotic product, the time for developing the product can be shortened. In a device (1) for estimating survival cell count, a calculation unit (14) calculates an estimated result of the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10 nt=Log10 n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)× 1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.

摘要翻译： 通过准确估计益生菌产品中的存活细胞计数，可以缩短开发产品的时间。 在用于估计存活细胞计数的装置（1）中，计算单元（14）根据以下等式计算储存后的组合物中包含的特定菌株的存活细胞计数nt（CFU / g）的估计结果 （一世）。 （I）Log10 nt = Log10 n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表保存期（天）×1/30，nt表示 储存期t（天）后组合物中所含的菌株的存活细胞计数（CFU / g），n0表示储存开始时组合物中包含的菌株的活菌数（CFU / g），T 代表储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的菌株特异性系数，BT和DT代表对菌株特异性的实验测定常数。

公开(公告)号：US20100310673A1

公开(公告)日：2010-12-09

申请号：US12867979

申请日：2009-03-31

申请人： Ayako Horigome ,  Mai Murata ,  Koji Yamauchi ,  Mitsunori Takase ,  Yasuhiro Takeda ,  Junichi Hashimoto ,  Ikumi Kojima

发明人： Ayako Horigome ,  Mai Murata ,  Koji Yamauchi ,  Mitsunori Takase ,  Yasuhiro Takeda ,  Junichi Hashimoto ,  Ikumi Kojima

IPC分类号： A61K33/34 ,  C07H21/00 ,  A61K33/26 ,  A61K33/30 ,  A61K38/40 ,  C07K14/79 ,  A61P31/04 ,  A61P3/00 ,  A61P37/04 ,  A61P29/00 ,  A23L1/305

CPC分类号： A61K47/42 ,  A23L33/19 ,  A61K9/0019 ,  A61K9/0095 ,  A61K9/08 ,  A61K9/2063 ,  A61K9/4866 ,  A61K38/40 ,  A61K47/26

摘要： An agent for thermally stabilizing lactoferrin, which comprises a nucleic acid as an active ingredient, can be added to lactoferrin to impart thermal stability to lactoferrin. Thermally stabilized lactoferrin can be heat-sterilized at a pH value around a neutral pH value while keeping its activity.

摘要翻译： 可以将含有核酸作为活性成分的用于热稳定的乳铁蛋白的试剂加入到乳铁蛋白中以赋予乳铁蛋白热稳定性。 热稳定的乳铁蛋白可以在pH值约为中性pH值的同时保持其活性而被热灭菌。

公开(公告)号：US09014990B2

公开(公告)日：2015-04-21

申请号：US12995066

申请日：2009-05-29

申请人： Fumiaki Abe ,  Ayako Uchijima ,  Masamichi Muto ,  Ayako Horigome

发明人： Fumiaki Abe ,  Ayako Uchijima ,  Masamichi Muto ,  Ayako Horigome

IPC分类号： G01N31/00 ,  C12Q1/06

CPC分类号： C12Q1/06

摘要： By accurately estimating the survival cell count in a probiotic product, the time for developing the product can be shortened. In a device (1) for estimating survival cell count, a calculation unit (14) calculates an estimated result of the survival cell count nt (CFU/g) of a specific strain contained in a composition after storage, in accordance with the following equation (I). (I) Log10nt=Log10n0−t×EXP{(AT×T+BT)w+(CT×T+DT)}, provided that t stands for the storage period (days)× 1/30, nt stands for the survival cell count (CFU/g) of the strain contained in the composition after the storage period t (days), n0 stands for the viable cell count (CFU/g) of the strain contained in the composition at the initiation of storage, T stands for the storage temperature (° C.), w stands for the water activity value of the composition, AT and CT stand for experimentally determined coefficients specific to the strain, and BT and DT stand for experimentally determined constants specific to the strain.

摘要翻译： 通过准确估计益生菌产品中的存活细胞计数，可以缩短开发产品的时间。 在用于估计存活细胞计数的装置（1）中，计算单元（14）根据以下等式计算储存后的组合物中包含的特定菌株的存活细胞计数nt（CFU / g）的估计结果 （一世）。 （I）Log10nt = Log10n0-t×EXP {（AT×T + BT）w +（CT×T + DT）}，条件是t代表储存期（天）×1/30，nt表示存活细胞 在储存期t（天）后，组合物中所含的菌株的计数（CFU / g），n0表示开始保存时组合物中包含的菌株的活菌数（CFU / g），T表示 储存温度（℃），w代表组合物的水活度值，AT和CT代表实验确定的应变特性系数，BT和DT代表对应变特异的实验测定常数。

公开(公告)号：US20120177624A1

公开(公告)日：2012-07-12

申请号：US13427242

申请日：2012-03-22

申请人： Kouichirou Shin ,  Ayako Horigome ,  Koji Yamauchi

发明人： Kouichirou Shin ,  Ayako Horigome ,  Koji Yamauchi

IPC分类号： A61K38/54 ,  A61P11/00 ,  A61P1/02

CPC分类号： A61K38/44 ,  A23C9/1213 ,  A23C13/16 ,  A23L29/06 ,  A23L33/10 ,  A61K31/19 ,  A61K31/194 ,  A61K31/198 ,  A61K31/7004 ,  A61K38/443 ,  C12Y101/03004 ,  C12Y111/01007 ,  A61K2300/00

摘要： Disclosed is a disinfectant which is safe, has few adverse side-effects, can be ingested together with a food or beverage by adding the disinfectant to the food or beverage, shows a bactericidal effect in the oral cavity, and has no problem even when ingested on a daily basis over a long period. Also disclosed is a food additive comprising the disinfectant. Specifically, disclosed is an oral disinfectant for the disinfection of an oral bacterium, which comprises lactoperoxidase, glucose oxidase, glucose and a pH-adjusting component.

摘要翻译： 公开了一种安全的消毒剂，具有很少的副作用，可以通过向食品或饮料中加入消毒剂与食物或饮料一起摄入，在口腔中显示杀菌作用，即使摄入也没有问题 长期以来每天都有。 还公开了包含消毒剂的食品添加剂。 具体地，公开了用于消毒口腔细菌的口腔消毒剂，其包括乳过氧化物酶，葡萄糖氧化酶，葡萄糖和pH调节组分。

公开(公告)号：US20100028274A1

公开(公告)日：2010-02-04

申请号：US12519585

申请日：2007-06-28

申请人： Kouichirou Shin ,  Ayako Horigome ,  Koji Yamauchi

发明人： Kouichirou Shin ,  Ayako Horigome ,  Koji Yamauchi

IPC分类号： A61K8/365 ,  A61K8/66 ,  A61P1/02

CPC分类号： A61K38/44 ,  A23C9/1213 ,  A23C13/16 ,  A23L29/06 ,  A23L33/10 ,  A61K31/19 ,  A61K31/194 ,  A61K31/198 ,  A61K31/7004 ,  A61K38/443 ,  C12Y101/03004 ,  C12Y111/01007 ,  A61K2300/00

摘要： Disclosed is a disinfectant which is safe, has few adverse side-effects, can be ingested together with a food or beverage by adding the disinfectant to the food or beverage, shows a bactericidal effect in the oral cavity, and has no problem even when ingested on a daily basis over a long period. Also disclosed is a food additive comprising the disinfectant. Specifically, disclosed is an oral disinfectant for the disinfection of an oral bacterium, which comprises lactoperoxidase, glucose oxidase, glucose and a pH-adjusting component.

摘要翻译： 公开了一种安全的消毒剂，具有很少的副作用，可以通过向食品或饮料中加入消毒剂与食物或饮料一起摄入，在口腔中显示杀菌作用，即使摄入也没有问题 长期以来每天都有。 还公开了包含消毒剂的食品添加剂。 具体地，公开了用于消毒口腔细菌的口腔消毒剂，其包括乳过氧化物酶，葡萄糖氧化酶，葡萄糖和pH调节组分。