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公开(公告)号：US20210324404A1
公开(公告)日：2021-10-21
申请号：US16967815
申请日：2018-12-28
发明人： Jian He , Bin Liu , Qian Peng , Qing Tao , Xiang Xiao , Xiaoming Bao
摘要： The present invention relates to an herbicide tolerance protein, an encoding gene thereof and use thereof, the herbicide tolerance protein comprising: a protein (a) having an amino acid sequence as shown in SEQ ID NO: 1, and having an alanine substitution at least at position 176 and/or having a valine substitution at position 178 of SEQ ID NO: 1; or (b) having an amino acid sequence as shown in SEQ ID NO: 3; or (c) having an amino acid sequence as shown in SEQ ID NO: 5; or (d) having an amino acid sequence as shown in SEQ ID NO: 7; or (e) being derived from (a) by means of the amino acid sequence of (a) undergoing substitution and/or deletion and/or by added one or several amino acids, and having the activity of thifensulfuron hydrolase. The herbicide tolerance protein of the present invention has a broad application prospects in plants.
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公开(公告)号：US20210246445A1
公开(公告)日：2021-08-12
申请号：US16972896
申请日：2019-05-29
发明人： Aihong ZHANG , Derong DING , Qing TAO , Xiaojiao LI
IPC分类号： C12N15/113 , A01N63/60 , C12N15/82
摘要： An isolated polynucleotide includes: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence comprising at least 15 or 17 or 19 or 21 contiguous nucleotides of SEQ ID NO: 1, wherein the ingestion, by Coleoptera insect pests, of a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence inhibits the growth of the Coleoptera insect pests; or (c) any one of the polynucleotide sequence as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence hybridized with the polynucleotide sequence defined in (a), (b) or (c) mentioned above under stringent conditions. Multiple target sequences of the target gene c46312 control the Coleoptera insect pest Monolepta hieroglyphica.
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公开(公告)号：US20210214744A1
公开(公告)日：2021-07-15
申请号：US15733802
申请日：2019-05-07
发明人： Aihong ZHANG , Derong DING , Qing TAO , Xiaojiao LI
IPC分类号： C12N15/82 , C12N15/113 , A01N63/60 , A01N25/02
摘要： Provided are an isolated polynucleotide and a method for controlling insect invasion. The isolated polynucleotide is a plurality of target sequences for controlling target gene c35112 of a coleopteran pest, Monolepta hieroglyphica, comprising: a) a polynucleotide sequence shown as SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15 or 17 or 19 or 21 contiguous nucleotides of SEQ ID NO: 1, a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence being capable of inhibiting the growth of coleopteran pests after being ingested by the coleopteran pests; or (c) any one of polynucleotide sequences shown as SEQ ID NO: 3 to SEQ ID NO: 20; or (d) a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence as defined in (a), (b) or (c).
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公开(公告)号：US20190106705A1
公开(公告)日：2019-04-11
申请号：US16086298
申请日：2016-12-09
发明人： Xiangting XIE , Qing TAO , Xiaoming BAO
IPC分类号： C12N15/82 , C07K14/395 , C12N9/14
摘要： A herbicide tolerant protein SUM1, a coding gene thereof and a use thereof, wherein the herbicide tolerant protein comprises: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein which is derived from (a) by substituting and/or deleting and/or adding one or more amino acids in the amino acid sequence of (a), and has the activity of thifensulfuron hydrolase. The herbicide tolerant protein SUM1 can show a higher tolerance to a plurality of sulfonylurea herbicides, can tolerate four-fold field concentration, and thus has a broad application prospect in plants.
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公开(公告)号：US11913003B2
公开(公告)日：2024-02-27
申请号：US16610870
申请日：2018-04-13
发明人： Yanhua Liu , Yuanyuan Wang , Zhiwei Jia , Qingfang Song
IPC分类号： C12N15/82
CPC分类号： C12N15/821 , C12N15/8205 , C12N15/8278
摘要： The present invention relates to a method of improving soybean transformation efficiency, which comprises: transforming a plant cell using a recombinant vector containing a gene of interest and a gene encoding a sulfonylurea herbicide hydrolase; screening and culturing the transformed plant cell by external application of an ALS inhibitor, using the gene encoding the sulfonylurea herbicide hydrolase as a selective marker; selecting a plant cell that has not been killed and/or not been inhibited. The present invention firstly proposes that a selective agent is added to a proliferation medium and a differentiation medium in a manner of external application during plant transformation process, and optimizes the effective screening concentration range of the selective agent, so the transformation efficiency is remarkably increased, and the proportion of positive plants obtained in the progeny thereof is significantly increased; at the same time, the transgenic plants obtained by the transformation using the sulfonylurea herbicide hydrolase gene as a selective marker in the present invention have high commercial value, good resistance and genetic stability.
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公开(公告)号：US20230212602A1
公开(公告)日：2023-07-06
申请号：US18063081
申请日：2022-12-08
发明人： Tengyu CHANG , Qinyang WANG , Aihong ZHANG , Qing TAO
IPC分类号： C12N15/82
CPC分类号： C12N15/8286
摘要： Related is a use of an insecticidal protein. The insecticidal protein may be used to control Monolepta hieroglyphica (Motschulsky). A method for controlling the Monolepta hieroglyphica (Motschulsky) includes: allowing the Monolepta hieroglyphica (Motschulsky) to be at least in contact with an ACh1 protein. In the present application, the Monolepta hieroglyphica (Motschulsky) is controlled through producing the ACh1 protein that can kill the Monolepta hieroglyphica (Motschulsky) in bacteria and/or a plant in vivo.
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公开(公告)号：US11365425B2
公开(公告)日：2022-06-21
申请号：US16308852
申请日：2017-04-07
发明人： Jian He , Li Yao , Xingjun Jia , Xiangting Xie , Yechun Wu , Qing Tao , Derong Ding
IPC分类号： C12N15/82 , C12N5/10 , C12N9/06 , C07K14/415 , C12N15/62
摘要： Provided are a resistant protein for use in herbicide dicamba, encoding gene and application thereof, the gene comprising: (a) a nucleotide sequence of an amino acid sequence as shown in SEQ ID NO: 2; or (b) a nucleotide sequence which is complementary to the nucleotide sequence as defined by (a) under stringent conditions; or (c) a nucleotide sequence as shown in SEQ ID NO: 1.
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公开(公告)号：US10676741B2
公开(公告)日：2020-06-09
申请号：US16068090
申请日：2016-12-09
发明人： Derong Ding , Aihong Zhang
IPC分类号： C12N15/82 , C12N15/113 , A01N57/16
摘要： Disclosed are a nucleotide sequence and a method for controlling insect invasions, the isolated polynucleotide sequence comprising: (a) a polynucleotide sequence as shown in SEQ ID NO: 1, 2 or 3; or (b) a polynucleotide sequence hybridizing with the polynucleotide sequence defined in (a) under stringent conditions; or (c) a polynucleotide sequence having an identity of not less than 80% to the polynucleotide sequence defined in (a); or (d) a polynucleotide sequence of at least 19 consecutive nucleotides of the polynucleotide sequence defined in (a), wherein a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence, when ingested by an insect pest from Hemiptera, inhibits growth of the insect pest; or (e) a complementary sequence of the polynucleotide sequence as defined in (a), (b), (c) or (d). The three target sequences for controlling an insect pest from Hemiptera, Lygus, have the advantages of efficiency, specificity, convenience and low cost.
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公开(公告)号：US10655140B2
公开(公告)日：2020-05-19
申请号：US15550342
申请日：2016-02-02
发明人： Qing Tao , Yechun Wu , Xiaoguang Niu , Xiangting Xie , Jie Pang , Xiaoming Bao
摘要： Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein with the activity of herbicide-resistance which is derived from the amino acid sequence in (a) by replacing and/or deleting and/or adding one or several amino acids in the same. The herbicide-resistant protein of this invention is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides.
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公开(公告)号：US20180244732A1
公开(公告)日：2018-08-30
申请号：US15550656
申请日：2016-02-02
发明人： Qing Tao , Yechun Wu , Xiaoguang Niu , Derong Ding
IPC分类号： C07K14/415 , C12N15/82
摘要： Involved is a herbicide-resistant protein, coding gene and use thereof. The herbicide-resistant protein comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein with the activity of herbicide-resistance which is derived from the amino acid sequence in (a) by replacing and/or deleting and/or adding one or several amino acids in the same. The herbicide-resistant protein of this invention is especially suitable for expression in plants, with broad resistance spectrum to herbicides, especially to phenoxy auxin herbicides.
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