公开(公告)号：US20080193457A1

公开(公告)日：2008-08-14

申请号：US10567386

申请日：2004-07-30

申请人： Nikolaos Berntenis ,  Gerrit Buurman ,  Harald Kropshofer ,  Bernd Christian Mueller ,  Sebastian Thomas Spindeldreher ,  Anne Vogt ,  Werner Zolg

发明人： Nikolaos Berntenis ,  Gerrit Buurman ,  Harald Kropshofer ,  Bernd Christian Mueller ,  Sebastian Thomas Spindeldreher ,  Anne Vogt ,  Werner Zolg

IPC分类号： A61K39/395 ,  C07K7/00 ,  C07K14/00 ,  C07K16/18 ,  C12N15/11 ,  A61P43/00 ,  C12N15/00 ,  C12N5/06 ,  A61K38/00 ,  G01N33/00

CPC分类号： C07K14/4713 ,  C07K14/70539

摘要： The present invention provides novel naturally-processed MHC class II antigenic peptides; which originate from interferon-γ-inducible lysosomal thiol reductase, integrin beta-2, phosphatitylinositol-4,5-bisphosphate 3-kinase, urokinase-type plas-minogen activator, immunoglobulin heavy chain V-III region (VH26), DJ-1 protein, apolipoprotein B-100, 26S proteasome non-AT-Pase regulatory subunit 8, interleukin-1 receptor, fibromodulin, GM-CSF/IL-3/IL-5 receptor, sorting nexin 3, inter-α-trypsin inhibitor heavy chain H4, complement C4, complement C3 (α-chain), complement C3 (β-chain), SH3 domain-binding glutamic acid-rich-like protein 3, interleukin-4-induced protein 1, hemopexin, Hsc70-interacting protein, invariant chain (Ii), retinoic acid receptor responder protein 2, fibronectin, cathepsin B, tripeptidyl-peptidase II, legumain, platelet activating factor receptor, poly-alpha-2.8-sialyltrans-ferase, and ras-leated protein Rab-11B. Also provided are these antigenic peptides and the proteins they are derived from as markers for erosive and/or non-erosive RA. Moreover, these antigenic peptides linked to MHC class II molecules, antibodies reactive with said antigenic peptides, nucleic acids encoding said antigenic peptides, and nucleic acid constructs, host cells and methods for expressing said antigenic peptides are provided. The antigenic peptides of the invention can be used as markers in diagnosis of RA and in therapy as anti-RA vaccines.

摘要翻译： 本发明提供了新型的天然加工的MHC II类抗原肽; 其衍生自干扰素-γ诱导型溶酶体硫醇还原酶，整联蛋白β-2，磷脂酰肌醇-4,5-二磷酸3-激酶，尿激酶型血小板原激活物，免疫球蛋白重链V-III区（V < / - 26），DJ-1蛋白，载脂蛋白B-100,26S蛋白酶体非AT-Pase调节亚单位8，白细胞介素-1受体，纤维调节蛋白，GM-CSF / IL-3 / IL-5受体，分选nexin 3 ，α间胰蛋白酶抑制剂重链H4，补体C4，补体C3（α链），补体C3（β链），SH3结构域结合谷氨酸样蛋白3，白细胞介素-4诱导蛋白1 ，血凝素，Hsc70相互作用蛋白，不变链（Ii），视黄酸受体应答蛋白2，纤连蛋白，组织蛋白酶B，三肽基肽酶II，legumain，血小板激活因子受体，聚α-2.8-唾液酸转移酶和ras - 蛋白质Rab-11B。 还提供了这些抗原肽和它们作为侵蚀性和/或非侵蚀性RA的标记物衍生的蛋白质。 此外，提供了与MHC II类分子连接的这些抗原肽，与所述抗原肽反应的抗体，编码所述抗原肽的核酸和核酸构建体，宿主细胞和用于表达所述抗原肽的方法。 本发明的抗原肽可用作RA诊断和抗RA疫苗治疗中的标志物。