公开(公告)号：US20070077226A1

公开(公告)日：2007-04-05

申请号：US10559008

申请日：2004-05-28

申请人： Xinyuan Liu ,  Zifel Pei ,  Binghua Li ,  Jinfa Gu ,  Weiguo Zou ,  Lanying Sun

发明人： Xinyuan Liu ,  Zifel Pei ,  Binghua Li ,  Jinfa Gu ,  Weiguo Zou ,  Lanying Sun

IPC分类号： A61K48/00 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/38 ,  C12N2830/85 ,  Y02A50/473

摘要： The present invention disclosed a kind of gutless adenovirus vector and the construction method thereof. Two structural independent but functional related cassettes, the trans-activator (TA) and anti-tumor cassette, are both carried by the gutless vector. hTERT promoter restricts the expression of TA only in tumor cells, and RU486, associated with TA, regulates the expression of interesting gene: when needed, add the RU486 and the gene expression is on, and when not needed, remove the RU486 and the gene expression is off. Tumor-specificity and small molecule regulation of the vector spare the toxicity to the normal tissue caused by the foreign gene product and endow the gene's long lifetime expression in vivo. The vector of the present invention shows many advantages over traditional adenovirus vectors in targeting, gene regulation and expression lifetime.

摘要翻译： 本发明公开了一种无痛腺病毒载体及其构建方法。 两个结构独立但功能相关的盒，反式激活剂（TA）和抗肿瘤盒都由无味载体携带。 hTERT启动子限制了TA在肿瘤细胞中的表达，而与TA相关的RU486可调节有趣基因的表达：需要时，添加RU486，并且基因表达是开启的，如果不需要，可以去除RU486和基因 表情是关闭的 载体的肿瘤特异性和小分子调节使外源基因产物引起的正常组织的毒性丧失，并在体内赋予基因的长寿命表达。 本发明的载体在靶向，基因调控和表达寿命中显示出优于传统腺病毒载体的许多优点。