公开(公告)号：US20140155284A1

公开(公告)日：2014-06-05

申请号：US14064140

申请日：2013-10-26

申请人： Jørn Erland Koch ,  Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Sissel Juul ,  Kam Leong ,  Yi-Ping Ho ,  Felicie F. Andersen

发明人： Jørn Erland Koch ,  Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Sissel Juul ,  Kam Leong ,  Yi-Ping Ho ,  Felicie F. Andersen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6893 ,  Y02A50/58

摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are also provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

摘要翻译： 公开了用于鉴定微生物或感染性疾病的方法，包括从诸如人，动物，植物，食物，水或土壤的来源获得合适的样品。 所述方法还包括提供设计成与来自一种或多种微生物或感染因子的1型拓扑异构酶反应的定制的核酸底物，以及将所述底物与所述样品或来自样品的提取物或制剂 如果所述微生物或感染剂存在于样品中，则底物被所述拓扑异构酶加工。 还提供了检测酶特别是DNA修饰酶的微流控实施方法，以及用于检测细胞或表达所述酶的微生物的方法。 通过提供由该酶特异性靶向的核酸底物来检测该酶。

公开(公告)号：US09567649B2

公开(公告)日：2017-02-14

申请号：US14064140

申请日：2013-10-26

申请人： Jørn Erland Koch ,  Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Sissel Juul ,  Kam Leong ,  Yi-Ping Ho ,  Felicie F. Andersen

发明人： Jørn Erland Koch ,  Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Sissel Juul ,  Kam Leong ,  Yi-Ping Ho ,  Felicie F. Andersen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6893 ,  Y02A50/58

摘要： Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are also provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

摘要翻译： 公开了用于鉴定微生物或感染性疾病的方法，包括从诸如人，动物，植物，食物，水或土壤的来源获得合适的样品。 所述方法还包括提供设计成与来自一种或多种微生物或感染因子的1型拓扑异构酶反应的定制的核酸底物，以及将所述底物与所述样品或来自样品的提取物或制剂 如果所述微生物或感染剂存在于样品中，则底物被所述拓扑异构酶加工。 还提供了检测酶特别是DNA修饰酶的微流控实施方法，以及用于检测细胞或表达所述酶的微生物的方法。 通过提供由该酶特异性靶向的核酸底物来检测该酶。

公开(公告)号：US20150018228A1

公开(公告)日：2015-01-15

申请号：US14241442

申请日：2012-08-31

申请人： Jørn Erland Koch ,  Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Sissel Juul ,  Kam Leong ,  Megan Yi-Ping Ho ,  Felicie F. Andersen

发明人： Jørn Erland Koch ,  Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Sissel Juul ,  Kam Leong ,  Megan Yi-Ping Ho ,  Felicie F. Andersen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6893 ,  C12Q1/00 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/689 ,  G01N33/573 ,  Y02A50/58

摘要： Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

摘要翻译： 提供检测酶特别是DNA修饰酶的微流控实施方法，以及用于检测细胞或表达所述酶的微生物的方法。 通过提供由该酶特异性靶向的核酸底物来检测该酶。