公开(公告)号：US07015017B2

公开(公告)日：2006-03-21

申请号：US10032897

申请日：2001-10-25

申请人： Manfred Eigen ,  Andreas Schwienhorst ,  Christof Biebricher ,  Björn Lindemann ,  Esteban Domingo ,  John Holland ,  Karsten Henco

发明人： Manfred Eigen ,  Andreas Schwienhorst ,  Christof Biebricher ,  Björn Lindemann ,  Esteban Domingo ,  John Holland ,  Karsten Henco

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/11 ,  A61K48/00 ,  A61K2039/525 ,  C12N7/00 ,  C12N15/00 ,  C12N2740/16011 ,  C12N2795/18111 ,  Y10T436/143333

摘要： A process for instabilizing viral quasi-species-distributions under avoidance of resistance phenomena by replication of the nucleic acids of the viruses present in the quasi-species-distribution by of a defective replication system, a) whereby the defective replication system has a rate of misincorporation for nucleotides above the rate of misincorporation of the viral wild-type-replication system and, whereby the viruses are replicated by the replication system having the higher rate of misincorporation at least as effectively as it is done by the replication system of the wild-type virus, b) and/or negative influence of the replication of the consensus-sequence (nucleic acid sequence of the wild-type virus) in relation to other replicatable nucleic acids.

公开(公告)号：US06423516B1

公开(公告)日：2002-07-23

申请号：US08362604

申请日：1996-03-22

申请人： Manfred Eigen ,  Andreas Schwienhorst ,  Christof Biebricher ,  Björn Lindemann ,  Esteban Domingo ,  John Holland ,  Karsten Henco

发明人： Manfred Eigen ,  Andreas Schwienhorst ,  Christof Biebricher ,  Björn Lindemann ,  Esteban Domingo ,  John Holland ,  Karsten Henco

IPC分类号： C12P1934

CPC分类号： C12N15/11 ,  A61K48/00 ,  A61K2039/525 ,  C12N7/00 ,  C12N15/00 ,  C12N2740/16011 ,  C12N2795/18111 ,  Y10T436/143333

摘要： A process for instabilizing viral quasi-species-distributions under avoidance of resistance phenomena by replication of the nucleic acids of the viruses present in the quasi-species-distribution by of a defective replication system, a) whereby the defective replication system has a rate of misincorporation for nucleotides above the rate of misincorporation of the viral wild-type-replication system and, whereby the viruses are replicated by the replication system having the higher rate of misincorporation at least as effectively as it is done by the replication system of the wild-type virus, b) and/or negative influence of the replication of the consensus-sequence (nucleic acid sequence of the wild-type virus) in relation to other replicatable nucleic acids.

摘要翻译： 一种通过利用有缺陷的复制系统复制存在于准物种分布中的病毒的核酸来避免抗性现象来阻止病毒准物种分布的方法，其中缺陷复制系统的速率为 对于高于病毒野生型复制系统错配的速率的核苷酸的错配，并且其中病毒由具有较高错配率的复制系统复制至少与野生型复制系统的复制系统一样有效地复制， 类型病毒，b）和/或共有序列（野生型病毒的核酸序列）相对于其他可重复核酸的复制的负面影响。