公开(公告)号：US06323001B1

公开(公告)日：2001-11-27

申请号：US09013598

申请日：1998-01-26

申请人： John Londesborough ,  Outi Tunnela ,  Tapio Palva ,  Kjell-Ove Holmström ,  Björn Welin ,  Abul Mandal

发明人： John Londesborough ,  Outi Tunnela ,  Tapio Palva ,  Kjell-Ove Holmström ,  Björn Welin ,  Abul Mandal

IPC分类号： C12N1500

CPC分类号： C07K14/395 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/92 ,  C12N15/8245

摘要： Two nucleotide sequences encoding two different polypeptides found in yeast trehalose synthase have been isolated and cloned. A third polypeptide has been isolated from the enzyme and characterized, and a method is provided to isolate and clone the nucleotide sequence encoding this polypeptide. The coding sequences can be inserted into suitable vectors and used to transform host cells. The transformed cells will produce increased amounts of trehalose compared to the untransformed wild types and have increased tolerance to a variety of stresses, in particular to decreased availability of water. The invention may be used to improve the stress tolerance of organisms, to increase the storage life of foodstuffs and to produce trehalose economically on an industrial scale in an organism (e.g. baker's yeast) that is a traditional and safe foodstuff.

摘要翻译： 已经分离并克隆了在酵母海藻糖合成酶中发现的编码两种不同多肽的两个核苷酸序列。 从酶中分离出第三种多肽，并进行表征，并提供了分离和克隆编码该多肽的核苷酸序列的方法。 编码序列可以插入合适的载体并用于转化宿主细胞。 与未转化的野生型相比，转化的细胞将产生增加量的海藻糖，并且具有增加的对各种胁迫的耐受性，特别是降低水的可利用性。 本发明可用于改善生物体的胁迫耐受性，增加食品的储存寿命，并在工业规模上经济地生产海藻糖，其为传统和安全食品的生物（例如面包酵母）。