公开(公告)号：US08877022B2

公开(公告)日：2014-11-04

申请号：US13165464

申请日：2011-06-21

Applicant: Shiming Lin ,  Bor-Ching Sheu ,  Luan-Yin Chang ,  Chih-Chen Lin

Inventor： Shiming Lin ,  Bor-Ching Sheu ,  Luan-Yin Chang ,  Chih-Chen Lin

IPC: G01N27/327 ,  G01N33/543

CPC classification number: G01N33/5438 ,  G01N27/3276

Abstract: Embodiments of the present disclosure set forth a biosensor for detecting a target. One example sensor includes a first electrode. The first electrode includes a first electron conducting molecule and a first probe. The first probe includes a second electron conducting molecule. The first probe is configured to bind to the target of interest in solution. The first and second electron conducting molecules are different.

Abstract translation: 本公开的实施例提出了用于检测目标的生物传感器。 一个示例性传感器包括第一电极。 第一电极包括第一电子传导分子和第一探针。 第一探针包括第二电子传导分子。 第一个探针被配置为绑定到解决方案中的感兴趣的目标。 第一和第二电子传导分子是不同的。

公开(公告)号：US20100171487A1

公开(公告)日：2010-07-08

申请号：US12652543

申请日：2010-01-05

Applicant: Shiming Lin ,  Shih-yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

Inventor： Shiming Lin ,  Shih-yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

IPC: G01R1/06

CPC classification number: G01N33/5438

Abstract: A method of electrosensing an antigen in a test sample using a sensor is disclosed. The sensor has two electrodes electrically disconnected and physically separated from each other and a layer of antibody immobilized on the surface of at least one of the electrodes. The antibody has specific binding reactivity with the antigen. The method comprises tethering conductivity promotion molecules over and/or distributing between the antibody-populated electrodes for improving electrical conductivity characteristics across the two electrodes, and measuring electrically across the electrodes after the test sample comes into contact with the antibody-populated electrodes. The antibody captures the antigen present in the test sample thereby altering the improved electrical conductivity characteristic across the two electrodes in which an amount representative of the altering providing an indication for electrosensing of the antigen.

Abstract translation: 公开了使用传感器对测试样品中的抗原进行电感应的方法。 传感器具有两个电极彼此电断开并物理分离，并且固定在至少一个电极的表面上的一层抗体。 抗体与抗原具有特异性结合活性。 该方法包括在抗体填充的电极之间和/或分布之间连接导电促进分子，以改善两个电极之间的导电特性，以及在测试样品与抗体填充的电极接触之后跨电极测量。 抗体捕获存在于测试样品中的抗原，从而改变跨越两个电极的改善的导电特性，其中代表改变的量提供抗原电感应的指示。

公开(公告)号：US20100170792A1

公开(公告)日：2010-07-08

申请号：US12652579

申请日：2010-01-05

Applicant: Shiming Lin ,  Shih-yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

Inventor： Shiming Lin ,  Shih-yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

IPC: G01N27/327 ,  C25B11/04

CPC classification number: G01N33/5438

Abstract: A sensor for electrosensing an antigen in a test sample is disclosed. The sensor has two electrodes electrically disconnected and physically separated from each other, and a layer of antibody is immobilized on the surface of at least one of the electrodes. The antibody has specific binding reactivity with the antigen. Conductivity promotion molecules may be tethered over and/or distributed between the antibody-populated electrodes for improving electrical conductivity characteristics across the two electrodes. The antibody captures the antigen present in the test sample mixed in a buffer solution that comes into contact with the antibody-populated electrodes. This alters the electrical conductivity characteristic across the two electrodes in which an amount representative of the altering provides an indication for electrosensing of the antigen.

Abstract translation: 公开了一种用于在测试样品中电敏感抗原的传感器。 传感器具有两个彼此电断开并物理分离的电极，并且一层抗体被固定在至少一个电极的表面上。 抗体与抗原具有特异性结合活性。 电导率促进分子可以束缚在抗体填充的电极之间和/或分布在抗体填充的电极之间，以改善跨两个电极的导电特性。 抗体捕获与在与抗体填充的电极接触的缓冲溶液中混合的测试样品中存在的抗原。 这改变了跨越两个电极的电导率特征，其中代表改变的量提供了抗原电感应的指示。

公开(公告)号：US20100170788A1

公开(公告)日：2010-07-08

申请号：US12652497

申请日：2010-01-05

Applicant: Shiming Lin ,  Shih-yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

Inventor： Shiming Lin ,  Shih-yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

IPC: G01N27/327

CPC classification number: G01N33/5438

Abstract: A sensor system for electrosensing an antigen in a test sample is disclosed. The sensor system has two electrodes electrically disconnected and physically separated from each other, and a layer of antibody is immobilized on the surface of the electrodes. The antibody has specific binding reactivity with the antigen. Conductivity promotion molecules suspended in a buffer solution may be distributed over and/or between the antibody-populated electrodes for improving electrical conductivity characteristics across the two electrodes. The antibody captures the antigen present in the test sample mixed in the buffer solution that comes into contact with the antibody-populated electrodes. This alters the electrical conductivity characteristic across the two electrodes in which an amount representative of the altering provides an indication for electrosensing of the antigen.

Abstract translation: 公开了一种用于对测试样品中的抗原进行电感应的传感器系统。 传感器系统具有两个彼此电隔离并物理分离的电极，并且一层抗体固定在电极的表面上。 抗体与抗原具有特异性结合活性。 悬浮在缓冲溶液中的电导率促进分子可以分布在抗体填充的电极之上和/或之间，以改善两个电极之间的导电特性。 抗体捕获混合在与抗体填充电极接触的缓冲溶液中的测试样品中存在的抗原。 这改变了跨越两个电极的电导率特征，其中代表改变的量提供了抗原电感应的指示。

公开(公告)号：US20100172800A1

公开(公告)日：2010-07-08

申请号：US12652419

申请日：2010-01-05

Applicant: Shiming Lin ,  Shih-Yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

Inventor： Shiming Lin ,  Shih-Yuan Lee ,  Bor-ching Sheu ,  Chih-chen Lin ,  Panchien Lin

IPC: G01N30/00

CPC classification number: G01N33/5438

Abstract: A sensor for electrosensing an antigen in a test sample is disclosed. The sensor has two electrodes electrically disconnected and physically separated from each other, a layer of antibody immobilized on the surface of at least one of said electrodes. The antibody has specific binding reactivity with the antigen. Conductivity promotion molecules are conjugated with the antibody to improve electrical conductivity characteristics across the two electrodes. The antibody captures the antigen present in the test sample mixed in a buffer solution that comes into contact with the antibody-populated electrodes. This alters the electrical conductivity characteristic across the two electrodes in which an amount representative of the altering provides an indication for electrosensing of the antigen.

Abstract translation: 公开了一种用于在测试样品中电敏感抗原的传感器。 该传感器具有彼此电隔离并物理分离的两个电极，一层抗体固定在至少一个所述电极的表面上。 抗体与抗原具有特异性结合活性。 电导率促进分子与抗体缀合以改善两个电极之间的导电特性。 抗体捕获与在与抗体填充的电极接触的缓冲溶液中混合的测试样品中存在的抗原。 这改变了跨越两个电极的电导率特征，其中代表改变的量提供了抗原电感应的指示。