公开(公告)号：US20010034433A1

公开(公告)日：2001-10-25

申请号：US09865578

申请日：2001-05-29

申请人： Celtrix Pharmaceuticals, Inc.

发明人： Emerald Martin Spencer ,  Carol Talkington-Verser

IPC分类号： C07H021/04 ,  C12P021/02 ,  A61K038/17 ,  C07K014/705

CPC分类号： C07K14/4743 ,  A61K38/00 ,  Y02A50/473

摘要： This invention relates to polypeptides that are human somatomedin carrier protein subunits and to processes for producing them. The carrier protein subunits bind to human somatomedin-like polypeptides, also known as insulin-like growth factors. The process involves preparation from a human serum fraction, Cohn IV-1, by a molecule of various chromatographic steps. This invention also relates to DNA molecules encoding human somatomedin carrier protein-like polypeptides, recombinant DNA molecules, hosts, processes for producing carrier protein-like polypeptides, human somatomedin carrier protein-like polypeptides produced using those molecules, hosts and processes. The invention relates to DNA molecules and their expression in appropriate hosts. The recombinant DNA molecules contain DNA molecules that code for polypeptides which have a biological activity of the human carrier protein or a human carrier protein subunit capable of binding somatomedins. The DNA molecules, recombinant DNA molecules, hosts, and processes of this invention may be used in the production of polypeptides useful in a variety of therapeutic, diagnostic, and other useful applications.

摘要翻译： 本发明涉及作为人生长调节素载体蛋白亚基的多肽及其生产方法。 载体蛋白亚基结合人类生长调节素样多肽，也称胰岛素样生长因子。 该方法包括通过各种色谱步骤的分子由人血清级分Cohn IV-1制备。 本发明还涉及编码人生长调节素载体蛋白样多肽的DNA分子，重组DNA分子，宿主，产生载体蛋白样多肽的方法，使用这些分子产生的人类生长调节蛋白载体蛋白样多肽，宿主和过程。 本发明涉及DNA分子及其在适当宿主中的表达。 重组DNA分子包含编码具有人载体蛋白的生物活性的多肽的DNA分子或能够结合生长调节素的人载体蛋白亚基。 本发明的DNA分子，重组DNA分子，宿主和方法可用于制备可用于多种治疗，诊断和其它有用应用的多肽。

公开(公告)号：US20020072589A1

公开(公告)日：2002-06-13

申请号：US09986944

申请日：2001-11-13

申请人： Celtrix Pharmaceuticals, Inc.

发明人： Desmond Mascarenhas ,  David Passmore ,  Stephen Danko

IPC分类号： C07K016/22

CPC分类号： C07K14/4743 ,  C07K2319/00

摘要： The invention relates to variants of native insulin-like growth factor binding protein 3 (IGFBP-3). Variant IGFBP-3s are disclosed which are modified to be resistant to hydrolysis. Also disclosed are variant IGFBP-3s where the nuclear localization signal (NLS) in native IGFBP-3 is altered. Additionally, amino-terminally extended IGFBP-3s are disclosed which include a variety of N-terminal extensions, including peptide and nucleotide binding domains, specific binding members such as ligand binding domains from receptors or antigen binding domains from immunoglobins, and peptide and protein hormones and growth factors. N-terminally extended IGFBP-3s may comprise hydrolysis-resistant or NLS variant IGFBP-3s.

摘要翻译： 本发明涉及天然胰岛素样生长因子结合蛋白3（IGFBP-3）的变体。 公开了修饰为耐水解的变体IGFBP-3。 还公开了其中天然IGFBP-3中的核定位信号（NLS）改变的变体IGFBP-3。 另外，公开了氨基末端延长的IGFBP-3，其包括多种N-末端延伸，包括肽和核苷酸结合结构域，特异性结合成员，例如来自免疫球蛋白的受体或抗原结合结构域的配体结合结构域，以及肽和蛋白激素 和生长因子。 N末端延长的IGFBP-3可以包含耐水解抗体或NLS变体IGFBP-3。