公开(公告)号：US08478669B1

公开(公告)日：2013-07-02

申请号：US11264049

申请日：2005-11-02

申请人： Christopher Coates ,  Robert Colamussi ,  Louis Botte ,  Robert T. Johnson ,  Cesar Davila ,  Laura Jaquez ,  Jon Baggett ,  Vanessa Bell ,  James Hassenzahl ,  James Gunther ,  Nancy Matt ,  Elvira Djonovic ,  Michael Joedecke ,  Esther Fong ,  Fredrick Miller ,  Joan Engler ,  Dennis Adams

发明人： Christopher Coates ,  Robert Colamussi ,  Louis Botte ,  Robert T. Johnson ,  Cesar Davila ,  Laura Jaquez ,  Jon Baggett ,  Vanessa Bell ,  James Hassenzahl ,  James Gunther ,  Nancy Matt ,  Elvira Djonovic ,  Michael Joedecke ,  Esther Fong ,  Fredrick Miller ,  Joan Engler ,  Dennis Adams

IPC分类号： G06Q40/00

CPC分类号： H04L41/12 ,  G06Q30/02 ,  H04B7/18539 ,  H04B7/18541 ,  H04B7/1856 ,  H04B7/18563 ,  H04W84/06

摘要： In one of many possible embodiments, a system is provided for modeling telecommunication switch investments. The exemplary system includes a telecommunication switch modeling application configured to determine equipment quantities having capacity to satisfy switch design parameters descriptive of at least one model telecommunication switch, and calculate model investments associated with the equipment quantities. The exemplary system also includes a telecommunication switch investment modeling application configured to weight the model investments in accordance with at least one of a network parameter descriptive of an actual telecommunication switch and a “what-if” input descriptive of a hypothetical telecommunication switch configuration to form weighted investments that are specific to at least one of the actual telecommunication switch and the hypothetical telecommunication switch configuration.

摘要翻译： 在许多可能的实施例之一中，提供了一种用于建模电信交换机投资的系统。 示例性系统包括电信交换机建模应用，其被配置为确定具有满足描述至少一个模型电信交换机的交换机设计参数的能力的设备数量，并且计算与设备数量相关联的模型投资。 该示例性系统还包括电信交换机投资建模应用，其被配置为根据描述实际电信交换机的网络参数和描述假设的电信交换机配置的“假设”输入中的至少一个来对模型投资进行加权以形成 特定于至少一个实际电信交换机和假设的电信交换机配置的加权投资。

公开(公告)号：US06228595B1

公开(公告)日：2001-05-08

申请号：US09619444

申请日：2000-07-19

申请人： Dale Lynn Morris ,  Julio Cesar Davila

发明人： Dale Lynn Morris ,  Julio Cesar Davila

IPC分类号： C12Q168

CPC分类号： C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q2600/142 ,  C12Q2600/158 ,  C12Q2537/143 ,  C12Q2521/107

摘要： Hepatocyte culturing system, primer sets and an analytical method for selectively detecting and quantitatively assessing the levels of mRNA expression of the major isoenzymes of cytochrome P450 (CYP450 1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2 and 4A1), fatty acyl-CoA oxidase (FACO) and select Phase II conjugating enzymes (UDPGT, GST and ST) in the rat using specific 5′ and 3′ oligonucleotide primers and reverse transcriptase-polymerase chain reaction. The method closely reproduces the expression obtained from rat liver tissue following treatment with the same enzyme inducers. Constitutive and inducible expression was maintained by resuspending, culturing and then overlaying adult rat hepatocytes with an extracellular matrix such as Matrigel®.

摘要翻译： 肝细胞培养系统，引物组和选择性检测和定量评估细胞色素P450主要同工酶（CYP450 1A1，1A2,2B1 / 2,2C11,2E1,3A2,3A2和4A1）的mRNA表达水平的分析方法，脂肪 酰基辅酶A氧化酶（FACO），并使用特异的5'和3'寡核苷酸引物和逆转录酶 - 聚合酶链反应在大鼠中选择II期缀合酶（UDPGT，GST和ST）。 该方法在用相同的酶诱导剂处理后，紧密地再现了从大鼠肝组织获得的表达。 通过用细胞外基质如Matrigel重悬，培养，然后覆盖成年大鼠肝细胞来保持组成型和诱导型表达。

公开(公告)号：US6127117A

公开(公告)日：2000-10-03

申请号：US855583

申请日：1997-05-13

申请人： Dale Lynn Morris ,  Julio Cesar Davila

发明人： Dale Lynn Morris ,  Julio Cesar Davila

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q2600/142 ,  C12Q2600/158

摘要： Hepatocyte culturing system, primer sets and an analytical method for selectively detecting and quantitatively assessing the levels of mRNA expression of the major isoenzymes of cytochrome P450 (CYP450 1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2 and 4A1), fatty acyl-CoA oxidase (FACO) and select Phase II conjugating enzymes (UDPGT, GST and ST) in the rat using specific 5' and 3' oligonucleotide primers and reverse transcriptase-polymerase chain reaction. The method closely reproduces the expression obtained from rat liver tissue following treatment with the same enzyme inducers. Constitutive and inducible expression was maintained by resuspending, culturing and then overlaying adult rat hepatocytes with an extracellular matrix such as Matrigel.RTM..

摘要翻译： 肝细胞培养系统，引物组和选择性检测和定量评估细胞色素P450主要同工酶（CYP450 1A1，1A2,2B1 / 2,2C11,2E1,3A2,3A2和4A1）的mRNA表达水平的分析方法，脂肪 酰基辅酶A氧化酶（FACO），并使用特异的5'和3'寡核苷酸引物和逆转录酶 - 聚合酶链反应在大鼠中选择II期缀合酶（UDPGT，GST和ST）。 该方法在用相同的酶诱导剂处理后，紧密地再现了从大鼠肝组织获得的表达。 通过用细胞外基质如Matrigel TM重悬，培养，然后覆盖成年大鼠肝细胞来维持组成型和诱导型表达。