公开(公告)号：US20240360507A1

公开(公告)日：2024-10-31

申请号：US18684929

申请日：2021-12-30

申请人： GENESPECTOR S.R.O.

发明人： Stanislav KMOCH ,  Lenka PIHEROVA ,  Hana HARTMANNOVA ,  Michal POHLUDKA ,  Martin RADINA

IPC分类号： C12Q1/6876 ,  C12Q1/6851

CPC分类号： C12Q1/6876 ,  C12Q1/6851 ,  C12Q2600/106 ,  C12Q2600/158

摘要： Method for predicting a severity of an infectious disease and biomarker for use in carrying out the method and monitoring a therapy of an infectious disease using an RT-qPCR method, while the method is being performed on a nasopharyngeal swab sample determining the amount of serum amyloid A mRNA, preferably SAA1, and the amount of serum amyloid A mRNA is normalized to the amount of mRNA of a constitutively expressed gene, which is preferably UBC. Based on the determined normalized value of the amount of SAA1 mRNA in the sample, the severity of the course of an infectious disease, which may be of viral, bacterial, or fungal origin, is predicted, and the effectiveness of a therapy of the given disease is further monitored.

公开(公告)号：US20240352523A1

公开(公告)日：2024-10-24

申请号：US18759368

申请日：2024-06-28

申请人： ENUMERIX, INC.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N2021/6439

摘要： This disclosure provides for devices, methods, and systems for performing a non-invasive prenatal testing (NIPT) digital assay upon generating at least a large number of counts per chromosome for a set of chromosomes present in a sample, where performing the NIPT digital assay can include: distributing nucleic acids of the sample and materials for an amplification reaction across a plurality of partitions; amplifying the nucleic acids with the materials, within the plurality of partitions; and generating counts per chromosome upon detecting signals from the plurality of partitions. The inventions enable processing of samples for NIPT digital analyses and/or other digital analyses involving other loci of interest, with unprecedented partitioning, reaction, readout, and analytical performance.

公开(公告)号：US20240352522A1

公开(公告)日：2024-10-24

申请号：US18685434

申请日：2022-08-23

申请人： CAMBRIDGE ENTERPRISE LIMITED

发明人： Ulrich KEYSER ,  Filip BOSKOVIC

IPC分类号： C12Q1/6876 ,  C12Q1/44 ,  C12Q1/6825 ,  C12Q1/70

CPC分类号： C12Q1/6876 ,  C12Q1/44 ,  C12Q1/6825 ,  C12Q1/701 ,  G01N2333/922

摘要： This invention relates to methods for detecting the presence or absence of target nucleic acids in samples by excising and detecting specific target probes.

公开(公告)号：US20240344127A1

公开(公告)日：2024-10-17

申请号：US18632071

申请日：2024-04-10

申请人： CFD Research Corporation

发明人： David Edwin Gaddes, III ,  Kyleigh Raye Jones

IPC分类号： C12Q1/6876 ,  C12Q1/6853

CPC分类号： C12Q1/6876 ,  C12Q1/6853 ,  C12Q2600/16

摘要： A method of performing an asymmetric loop-mediated isothermal amplification (LAMP) assay can include: providing the asymmetrical primer set of one of the embodiments; providing a reagent composition having a polymerase; combining the asymmetrical primer set and reagent composition with a sample having the target nucleic acid to form an amplification mixture; heating the amplification mixture to an amplification temperature within an amplification temperature range; maintaining the amplification temperature to amplify the target nucleic acid having the target sequence; and obtaining an amplified amount of the target nucleic acid having the target sequence. The asymmetrical primer set can include one primer of a primer pair to be at a lower (e.g., 10%) amount of the other primer.

公开(公告)号：US12116630B2

公开(公告)日：2024-10-15

申请号：US16948526

申请日：2020-09-22

申请人： Boise State University

发明人： Stephanie F. Hudon ,  Steven Burden ,  Eric Hayden ,  Esteban Palencia Hurtado

IPC分类号： C12Q1/68 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12Q2600/124 ,  C12Q2600/166

摘要： Disclosed herein are methods of determining telomere length using universal reference primers. The reference primer pairs efficiently and reproducibly amplify genomic DNA in any vertebrate species. Kits for determining the length of telomeres or others repetitive regions in a sample are also provided.

公开(公告)号：US20240336962A1

公开(公告)日：2024-10-10

申请号：US18294286

申请日：2022-08-05

申请人： Agency for Science, Technology and Research

发明人： Lian Chye Winston Koh ,  Kiat Whye Kong

IPC分类号： C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/6876

CPC分类号： C12Q1/6851 ,  C12Q1/6876 ,  C12Q1/6806

摘要： This disclosure relates to a method of amplification of a target nucleic acid, the method comprising subjecting the target nucleic acid to one or more amplification step in the presence of a mixture comprising a control nuclei acid, a surfactant and an oligonucleotide primer and/or probe capable of hybridizing with the target nucleic acid, wherein the oligonucleotide primer and/or probe comprises a cleavage site and a cleavable 3′ end. In one embodiment, the target nucleic acid is cell-free RNA and the method comprises annealing the target nucleic acid and performing a reverse transcription prior to the one or more amplification step. Also disclosed are nucleic acid amplification mixtures, kits for amplifications, and methods of detecting and/or determining the presence and/or the amount of a target nucleic acid.

公开(公告)号：US20240327907A1

公开(公告)日：2024-10-03

申请号：US18439062

申请日：2024-02-12

申请人： Singular Genomics Systems, Inc.

发明人： Ronald Graham ,  Megha Cila

IPC分类号： C12Q1/6874 ,  C07H19/10 ,  C07H19/20 ,  C12Q1/6806 ,  C12Q1/6876

CPC分类号： C12Q1/6874 ,  C07H19/10 ,  C07H19/20 ,  C12Q1/6876 ,  C12Q1/6806

摘要： Disclosed herein, inter alia, are methods and cleavable compounds that minimize byproduct formation following cleavage.

公开(公告)号：US12104208B2

公开(公告)日：2024-10-01

申请号：US16617980

申请日：2018-05-31

申请人： BLUE GENOMICS CHILE SPA

发明人： Daniela Cichero Molina ,  Jorgen Odegard ,  Sven Korsvoll

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12Q2600/124 ,  C12Q2600/156

摘要： The invention relates to methods of predicting resistance to Piscirickettsia salmonis infection in a salmonid, the method comprising determining in the salmonid the alleles present at one or more DNA polymorphism within a QTL, and predicting the ability of the salmonid to be resistant to Piscirickettsia salmonis infection based on the determination of the alleles, wherein the QTL is: —(a) located in linkage group 21 (GenBank ID NC 034194.1) within the coho salmon genome, or in the chromosome of coho salmon that corresponds to that linkage group, when the salmonid is a coho salmon, or; (b) a QTL that is located in a linkage group that corresponds to linkage group 21 within the coho salmon genome, or in the chromosome of a salmonid that corresponds to that linkage group, when the salmonid is not a coho salmon. The invention further relates to probes and arrays useful in such method and related methods.

公开(公告)号：US20240318264A1

公开(公告)日：2024-09-26

申请号：US18652730

申请日：2024-05-01

申请人： Becton, Dickinson and Company

发明人： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC分类号： C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC分类号： C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16

摘要： The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US12098368B2

公开(公告)日：2024-09-24

申请号：US17737880

申请日：2022-05-05

申请人： Cepheid

发明人： Russell Higuchi

IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12P19/34 ,  C12Q1/6876

CPC分类号： C12N15/11 ,  C12P19/34 ,  C12Q1/6876 ,  C12N2310/321 ,  C12N2310/333 ,  C12N2310/334 ,  C12N2310/335 ,  C12N2310/336 ,  C12N2310/531

摘要： Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a 3-fold or greater increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Modified bases can be employed in primers to provide this base-3 or greater amplification with satisfactory PCR cycle times, which are improved, as compared to those observed in the absence of modified bases.