公开(公告)号：US20140295426A1

公开(公告)日：2014-10-02

申请号：US14235185

申请日：2012-07-27

申请人： Steven M. Albelda ,  Daniel Schwed ,  Chandra Galla Rao ,  Mark Connelly ,  Brad Foulk

发明人： Steven M. Albelda ,  Daniel Schwed ,  Chandra Galla Rao ,  Mark Connelly ,  Brad Foulk

IPC分类号： G01N33/574 ,  C12Q1/68

CPC分类号： G01N33/574 ,  C12Q1/6841 ,  G01N1/40 ,  G01N1/4077 ,  G01N33/57488 ,  G01N2333/705

摘要： A method for diagnosing or differentially diagnosing a cancer characterized by the presence of cancer cells in the pleural fluid of a mammalian subject, the method comprising contacting a sample of pleural fluid of the subject with colloidal magnetic particles coupled to a ligand which binds to a determinant on a cancer cell, but does not bind above a baseline threshold to other cellular and non-cellular components in pleural fluid; subjecting the pleural fluid-magnetic particle mixture to a magnetic field to produce a cell fraction enriched in ligand coupled-magnetic particle-bound cancer cells, if present in the pleural fluid; and analyzing the enriched fraction for the number of cancer cells in the pleural fluid. In certain aspects, this method involves preparing the pleural fluids for the above-noted method steps by, e.g., dilution of unprocessed pleural fluid. In certain aspect, the pleural fluid is subjected to the diagnostic method within 24 hours of withdrawal from the subject. This method has advantages to present diagnostic procedures for identifying malignant pleural effusions. The tumor cells present in pleural fluid can be characterized with cellular and molecular markers to determine prognostic and predictive factors.

摘要翻译： 一种用于诊断或差异诊断癌症的方法，其特征在于哺乳动物受试者的胸膜液中存在癌细胞，所述方法包括使受试者的胸膜液样品与结合至决定簇的配体偶联的胶体磁性颗粒接触 但不能在胸膜液中的其他细胞和非细胞成分的基线阈值以上结合; 使胸膜液 - 磁性颗粒混合物进行磁场以产生富含配体偶联磁性颗粒结合的癌细胞（如果存在于胸膜液中）的细胞部分; 并分析胸膜液中癌细胞数量的富集部分。 在某些方面，该方法包括通过例如稀释未加工的胸膜液来制备上述方法步骤的胸膜液。 在某些方面，在从受试者撤出的24小时内对胸膜液进行诊断。 该方法具有鉴定恶性胸腔积液的诊断方法的优点。 存在于胸膜液中的肿瘤细胞可以用细胞和分子标记进行表征，以确定预后和预测因素。