公开(公告)号：US07615369B2

公开(公告)日：2009-11-10

申请号：US11233975

申请日：2005-09-22

申请人： Chih-Tin Lin ,  Amaya Frost ,  Edgar Meyhöfer ,  Katsuo Kurabayashi ,  Chao Yung Fan

发明人： Chih-Tin Lin ,  Amaya Frost ,  Edgar Meyhöfer ,  Katsuo Kurabayashi ,  Chao Yung Fan

IPC分类号： C12M1/34

CPC分类号： G01N33/5438 ,  G01N33/54306

摘要： A protein patterning electrode device consisting of capacitor microelectrode arrays coated with a protein non-adherent layer is provided. Operation of the electrode is based on a phenomenon called “electrowetting,” where surface wettability can dynamically be controlled by varying the voltage across the device electrodes. When an electric field is applied across the electrode layers, the surface accumulates charge and becomes hydrophilic, binding the proteins to the surface via ionic bonding. Electrically controlling the amount of the surface charge permits controlled protein surface affinity. The device provides a means for reconfigurable protein patterning.

摘要翻译： 提供了由涂覆有蛋白质非粘附层的电容器微电极阵列组成的蛋白质图案形成电极装置。 电极的操作基于称为“电润湿”的现象，其中通过改变器件电极两端的电压来动态地控制表面润湿性。 当跨越电极层施加电场时，表面积累电荷并变成亲水性，通过离子键将蛋白质结合到表面。 电子控制表面电荷的量允许控制的蛋白质表面亲和力。 该装置提供了可重构蛋白质图案化的手段。