公开(公告)号：US20070298496A1

公开(公告)日：2007-12-27

申请号：US11651563

申请日：2007-01-10

申请人： Hung-Chih Kuo ,  Ya-Ling Chen ,  Yu-Ting Yan ,  Chia-Ning Shen ,  Shu-Hwa Chen ,  John Yu ,  Ching-Yu Chuang

发明人： Hung-Chih Kuo ,  Ya-Ling Chen ,  Yu-Ting Yan ,  Chia-Ning Shen ,  Shu-Hwa Chen ,  John Yu ,  Ching-Yu Chuang

IPC分类号： C12N5/06

CPC分类号： C12N5/0606 ,  A61K35/12 ,  C12N5/0618 ,  C12N5/0657 ,  C12N5/067 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/06 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2510/00 ,  C12N2533/90

摘要： The present invention provides a high efficiency method of deriving pluripotent mammalian stem cells from single, dissociated blastomeres harvested from preimplantation embryos.

摘要翻译： 本发明提供了从植入前胚胎收获的单个解离的卵裂球中获得多能哺乳动物干细胞的高效方法。

公开(公告)号：US20220396798A1

公开(公告)日：2022-12-15

申请号：US17489357

申请日：2021-09-29

申请人： Shi-Lung LIN ,  Samantha CHANG-LIN ,  Jack SK CHEN ,  David TS WU ,  Chia-Ning SHEN ,  Mei-Jung WANG

发明人： Shi-Lung LIN ,  Samantha CHANG-LIN ,  Jack SK CHEN ,  David TS WU ,  Chia-Ning SHEN ,  Mei-Jung WANG

IPC分类号： C12N15/113 ,  C12N15/85

摘要： This invention relates to a novel mRNA composition and its production method useful for developing and manufacturing RNA-based anti-viral and/or anti-cancer vaccines and medicines. This invention includes two types of mRNA constructs, namely “5′-hairpin messenger RNA (5hmRNA)” and “messenger-hairpin-messenger RNA (mhmRNA)”, respectively. Both of 5hmRNA and mhmRNA contain at least a hairpin-like stem-loop RNA structure. The 5hmRNA contains at least a stem-loop RNA structure in the 5′-UTR of a protein/peptide-coding mRNA, while the mhmRNA contains a middle stem-loop structure flanked with two protein/peptide-coding mRNA sequences on both sides. In mhmRNA, the first 5′-mRNA preferably encodes an RNA replicase, for amplifying the second 3′-mRNA in transfected cells. After transfection into target cells, 5hmRNA and mhmRNA can be further translated into at least a desired protein/peptide. To produce highly structured 5hmRNA and mhmRNA, a novel PCR-IVT methodology has been developed and used with a specially designed RNA polymerase-helicase mixture reaction