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公开(公告)号：US12098389B2
公开(公告)日：2024-09-24
申请号：US17844757
申请日：2022-06-21
申请人： KEIO UNIVERSITY
发明人： Minoru Ko
IPC分类号： C12N5/071 , C12N5/077 , C12N5/0793 , C12N15/09 , C12N15/113
CPC分类号： C12N5/0658 , C12N5/0619 , C12N5/067 , C12N15/09 , C12N15/113 , C12N2310/14 , C12N2506/02
摘要： In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.
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公开(公告)号：US20240300977A1
公开(公告)日：2024-09-12
申请号：US18287227
申请日：2022-04-15
发明人： Micah Maetani , Xin Jenny Xie , Anthony Forget , Aaron Prodeus , Stephanie Yazinski , Rubina Giare Parmar
IPC分类号： C07D519/00 , C12N5/071 , C12N5/0783 , C12N9/22 , C12N15/113 , C12N15/90
CPC分类号： C07D519/00 , C12N5/0636 , C12N5/067 , C12N9/22 , C12N15/113 , C12N15/907 , C12N2310/20 , C12N2510/00
摘要： The present disclosure relates to inhibitors of DNA protein kinase, and compositions and methods of use thereof. In some embodiments, the inhibitors have the structure of Formula I:
or a salt thereof,
wherein:
x1 is C—R3 or N;
R1 is C1-C3 alkyl;
R2 is cycloalkyl or heterocyclyl, and cycloalkyl and heterocyclyl are optionally substituted with one or more R6;
R3 is H or C1-C3 alkyl;
R4 is H or C1-C3 alkyl;
R5 is C1-C3 alkyl;
each R6 is independently selected from hydroxy, halo, alkyl, alkoxy, cycloalkyl, amino, and cyano, or two R6, taken together with the atom or atoms to which they are bonded, form a spirocyclic or fused ring; and
R7 is H or C1-C3 alkyl.-
公开(公告)号：US20240279597A1
公开(公告)日：2024-08-22
申请号：US18603745
申请日：2024-03-13
发明人： Taito NISHINO , Tatsuro KANAKI , Ayako OTANI , Koichiro SARUHASHI , Misayo TOMURA , Takehisa IWAMA , Masato HORIKAWA , Norio NAKATSUJI , Tomomi OTSUJI
CPC分类号： C12N5/0043 , C12N5/005 , C12N5/067 , C12N5/0693 , G01N33/5011 , G01N33/5014 , G01N33/5044 , G01N33/5067 , C12N2500/14 , C12N2500/34 , C12N2531/00 , C12N2533/80
摘要： The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like
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公开(公告)号：US20240240201A1
公开(公告)日：2024-07-18
申请号：US18289516
申请日：2022-05-04
申请人： MOGRIFY LIMITED
IPC分类号： C12N15/86 , C12N5/071 , C12N5/077 , C12N5/0786 , C12N5/0793
CPC分类号： C12N15/86 , C12N5/0619 , C12N5/0645 , C12N5/0658 , C12N5/067 , C12N2506/1307 , C12N2506/45 , C12N2770/36143 , C12N2840/203
摘要： Described herein are methods and compositions containing self-replicating RNA encoding at least one transcription factor and their uses in cell conversion of a pluripotent stem cell. a multipotent stem cell. or a differentiated source cell to a desired target cell. The methods and compositions can be used to transfect human pluripotent or multipotent stem cells and various human somatic cell types with self- replicating RNA to convert cells in vitro, in vivo or ex vivo.
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公开(公告)号：US20240228951A1
公开(公告)日：2024-07-11
申请号：US18595503
申请日：2024-03-05
申请人： RXCELL INC.
发明人： Xianmin ZENG , Mahendra RAO
IPC分类号： C12N5/0735 , C12N5/071 , C12N5/0775 , C12N5/079 , C12N5/0793 , C12N5/0797
CPC分类号： C12N5/0606 , C12N5/0619 , C12N5/0621 , C12N5/0622 , C12N5/0623 , C12N5/0668 , C12N5/067 , C12N2506/03
摘要： Methods are provided for generating multiple cellular products via differentiation of cells from single clinically compliant pluripotent cells into multiple cellular products selected from retinal epithelium, retinal progenitors, neural stem cells, dopaminergic neurons, astrocytes, hepatocytes, endothelial cells and mesenchymal cells using standard differentiation protocols for the multiple cellular products.
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公开(公告)号：US20240182863A1
公开(公告)日：2024-06-06
申请号：US18531019
申请日：2023-12-06
IPC分类号： C12N5/071
CPC分类号： C12N5/067 , C12N2502/1323 , C12N2533/54 , C12N2533/90 , C12N2537/10
摘要： The present disclosure relates to fibers prepared using decellularized liver ECM. The disclosure further provides in vitro cultures of human hepatocytes on decellularized liver ECM fibers, and the use of the cultures in developing and screening drugs.
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公开(公告)号：US20240084260A1
公开(公告)日：2024-03-14
申请号：US18271715
申请日：2022-01-14
申请人： JSR CORPORATION
发明人： Norio MASUDA , Toshiro SATO
IPC分类号： C12N5/071
CPC分类号： C12N5/067 , C12N2501/15 , C12N2501/2306 , C12N2501/998
摘要： Provided is a method of culturing a human hepatic parenchymal cell, including culturing a human hepatic parenchymal cell in a medium containing: a culture supernatant of a human hepatic nonparenchymal cell; a Wnt signaling promoter; and a mitogenic growth factor.
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公开(公告)号：US20240010987A1
公开(公告)日：2024-01-11
申请号：US18038444
申请日：2021-11-24
发明人： Seong Wook SEO , Yoon Pyo CHOI , Sang Yeop AHN , Da In PARK , Myeong Hoon LEE
CPC分类号： C12N5/067 , C12M29/04 , C12N2503/00 , C12N2502/1157
摘要： The present invention relates to an in vitro liver disease model using triple co-culture and a preparation method thereof, and the method of preparing the in vitro liver disease model includes directly co-culturing hepatocytes and hepatic stellate cells, and simultaneously indirectly co-culturing Kupffer cells by being separated from the hepatocytes and the hepatic stellate cells to enable a rapid and highly accurate analysis by similarly implementing the in vivo environment compared to mono-culture, and simultaneously make it easier to collect and measure specimens than typical direct co-culture.
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公开(公告)号：US20230341378A1
公开(公告)日：2023-10-26
申请号：US18214651
申请日：2023-06-27
申请人： EMULATE, Inc.
发明人： Daniel Levner , Kyung Jin Jang , Jacob Fraser , S. Jordan Kerns , Antonio Varone , Dongeun Huh
IPC分类号： G01N33/50 , C12N5/071 , C12M1/12 , C12N5/077 , C12N5/00 , C07K2/00 , C07K14/78 , C12M3/06 , C12M1/00
CPC分类号： G01N33/5032 , C07K2/00 , C07K14/78 , C12M23/16 , C12M23/20 , C12M25/02 , C12N5/0018 , C12N5/0068 , C12N5/0658 , C12N5/067 , G01N33/5014 , C12N2500/32 , C12N2521/00 , C12N2533/30 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/90 , C12N2535/10 , C12N2537/10 , G01N33/5044
摘要： Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.
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公开(公告)号：US20230324359A1
公开(公告)日：2023-10-12
申请号：US18300582
申请日：2023-04-14
发明人： Ken-ichi YAMADA , Saki SHINTO , Tomomi IDE , Keiichi YAMAMOTO
摘要： The present invention provides: an assay method that uses a compound represented by formula (I) as a fluorescent probe molecule and that is for detecting the lipid peroxidation suppression activity of a test compound; an assay kit that uses the assay method; a screening method that uses the assay method; and a pharmaceutical composition that is for the treatment, etc. of diseases (such as age-related macular degeneration) that are induced by lipid peroxidation reactions.
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