公开(公告)号：US06307020B1

公开(公告)日：2001-10-23

申请号：US09117121

申请日：1998-11-20

申请人： Choy Hew ,  Zhiyuan Gong

发明人： Choy Hew ,  Zhiyuan Gong

IPC分类号： C07K100

CPC分类号： C12N15/8273 ,  C07K14/461

摘要： A family of related intracellular skin type antifreeze polypeptides and corresponding coding nucleic acids are provided. These are the first skin type intracellular antifreeze polypeptides and coding nucleic acids ever reported. The polypeptides are naturally expressed in the skin of Winter Flounder, and skin specific promoters are also provided. The polypeptides are used to make cells cold-resistant, and to improve the palatability of cold foods and liquids. Cold resistant eukaryotes and prokaryotes, including plants, animals and bacteria are made using the skin-type intracellular antifreeze polypeptides and nucleic acids.

摘要翻译： 提供了一系列相关的细胞内皮肤型防冻多肽和相应的编码核酸。 这些是曾经报道的第一种皮肤型细胞内抗冻多肽和编码核酸。 多肽在冬季比目鱼的皮肤中天然表达，并提供皮肤特异性启动子。 多肽用于使细胞耐寒，并提高冷食品和液体的适口性。 使用皮肤型细胞内抗冻多肽和核酸制备耐寒真核生物和原核生物，包括植物，动物和细菌。