公开(公告)号：US07700928B2

公开(公告)日：2010-04-20

申请号：US11627259

申请日：2007-01-25

申请人： Brian Rasnow ,  Chuck Z. Li

发明人： Brian Rasnow ,  Chuck Z. Li

IPC分类号： G01N21/64

CPC分类号： G01N21/6454

摘要： An apparatus is provided that is capable of interleaving detection of fluorescence and luminescence signals emitted from a plurality of samples. The apparatus is suitable for analysis of samples containing single cells or tissues up to and including living organisms. It contains an optical assembly or “sandwich” for producing a spectrally pure and spatially dispersed light source for illuminating the sample. The invention also provides a plurality of optical sandwiches that can be variously geometrically arranged and their intensities programmed to create spatially uniform illumination over a large sample. The invention further provides an apparatus having at least one of the optical sandwich and a detector system capable of interleaving detection of fluorescent and luminescent signals when a suitable sample is illuminated by the light source of the optical sandwich. Methods for preparing samples and using the sandwiches, arrays and apparatus, are further provided by this invention. A method for interleaving detection of fluorescent and luminescent signals emitted from a plurality of samples is disclosed.

摘要翻译： 提供一种能够交错检测从多个样品发射的荧光和发光信号的装置。 该装置适用于分析含有直至并包括活生物体的单个细胞或组织的样品。 它包含用于产生用于照射样品的光谱纯净和空间分散的光源的光学组件或“夹心”。 本发明还提供了多个光学三明治，其可以在各种几何上布置，并且其强度被编程以在大样品上产生空间均匀的照明。 本发明还提供一种具有光学三明治和检测器系统中的至少一个的装置，当由光学三明治的光源照射合适的样品时，能够交错检测荧光和发光信号。 本发明还提供了制备样品和使用三明治，阵列和装置的方法。 公开了一种用于交叉检测从多个样本发射的荧光和发光信号的方法。

公开(公告)号：US20080031781A1

公开(公告)日：2008-02-07

申请号：US11500174

申请日：2006-08-07

申请人： Brian Rasnow ,  Chuck Z. Li ,  Stephen Robert Wilson

发明人： Brian Rasnow ,  Chuck Z. Li ,  Stephen Robert Wilson

IPC分类号： B01L3/00

CPC分类号： B01L9/543 ,  B01L2300/0829 ,  G01N2035/103 ,  Y10T436/2575

摘要： A method of loading a plurality of pipette tips on a plurality of mandrels of a pipettor is provided. A plurality of pipette tips are inserted in a plurality of receptacles of a tip loading assembly. The plurality of pipette tips are oriented with respect to each other by a plate. The tip loading assembly includes, but is not limited to, an insertion surface. The insertion surface includes the plurality of receptacles configured to accept the plurality of pipette tips. A receptacle from among the plurality of receptacles includes a receptacle wall and a beveled surface that extends from at least a portion of the insertion surface. A retention force is applied to the plate. The plurality of pipette tips are attached to a plurality of mandrels of a pipettor and are separated from the plate.

摘要翻译： 提供了一种将多个移液管尖端装载在移液器的多个心轴上的方法。 多个移液管尖端插入到尖端装载组件的多个插座中。 多个吸管尖端通过板相对于彼此定向。 尖端装载组件包括但不限于插入表面。 插入表面包括被配置为接受多个移液管吸头的多个容器。 多个插座中的插座包括插座壁和从插入表面的至少一部分延伸的斜面。 保持力施加到板上。 多个移液器吸头附接到移液器的多个心轴并与板分离。

公开(公告)号：US20080179539A1

公开(公告)日：2008-07-31

申请号：US11627259

申请日：2007-01-25

申请人： Brian Rasnow ,  Chuck Z. Li

发明人： Brian Rasnow ,  Chuck Z. Li

IPC分类号： G01N21/64

CPC分类号： G01N21/6454

摘要： An apparatus is provided that is capable of interleaving detection of fluorescence and luminescence signals emitted from a plurality of samples. The apparatus is suitable for analysis of samples containing single cells or tissues up to and including living organisms. It contains an optical assembly or “sandwich” for producing a spectrally pure and spatially disperse light source for illuminating the sample. The invention also provides a plurality of optical sandwiches that can be variously geometrically arranged and their intensities programmed to create spatially uniform illumination over a large sample. The invention further provides an apparatus having at least one of the optical sandwich and a detector system capable of interleaving detection of fluorescent and luminescent signals when a suitable sample is illuminated by the light source of the optical sandwich. Methods for preparing samples and using the sandwiches, arrays and apparatus, are further provided by this invention. A method for interleaving detection of fluorescent and luminescent signals emitted from a plurality of samples is disclosed.

摘要翻译： 提供一种能够交错检测从多个样品发射的荧光和发光信号的装置。 该装置适用于分析含有直至并包括活生物体的单个细胞或组织的样品。 它包含用于产生用于照亮样品的光谱纯的和空间分散的光源的光学组件或“夹心”。 本发明还提供了多个光学三明治，其可以在各种几何上布置，并且其强度被编程以在大样品上产生空间均匀的照明。 本发明还提供一种具有光学三明治和检测器系统中的至少一个的装置，当由光学三明治的光源照射合适的样品时，能够交错检测荧光和发光信号。 本发明还提供了制备样品和使用三明治，阵列和装置的方法。 公开了一种用于交叉检测从多个样本发射的荧光和发光信号的方法。

公开(公告)号：US08012767B2

公开(公告)日：2011-09-06

申请号：US11500174

申请日：2006-08-07

申请人： Brian Rasnow ,  Chuck Z. Li ,  Stephen Robert Wilson

发明人： Brian Rasnow ,  Chuck Z. Li ,  Stephen Robert Wilson

IPC分类号： G01N1/10 ,  B01L3/02

CPC分类号： B01L9/543 ,  B01L2300/0829 ,  G01N2035/103 ,  Y10T436/2575

摘要： A method of loading a plurality of pipette tips on a plurality of mandrels of a pipetter is provided. A plurality of pipette tips are inserted in a plurality of receptacles of a tip loading assembly. The plurality of pipette tips are oriented with respect to each other by a plate. The tip loading assembly includes, but is not limited to, an insertion surface. The insertion surface includes the plurality of receptacles configured to accept the plurality of pipette tips. A receptacle from among the plurality of receptacles includes a receptacle wall and a beveled surface that extends from at least a portion of the insertion surface. A retention force is applied to the plate. The plurality of pipette tips are attached to a plurality of mandrels of a pipetter and are separated from the plate.

摘要翻译： 提供了一种将多个移液管吸头装载到移液器的多个心轴上的方法。 多个移液管尖端插入到尖端装载组件的多个插座中。 多个吸管尖端通过板相对于彼此定向。 尖端装载组件包括但不限于插入表面。 插入表面包括被配置为接受多个移液管吸头的多个容器。 多个插座中的插座包括插座壁和从插入表面的至少一部分延伸的斜面。 保持力施加到板上。 多个移液管尖端附接到多个移液器的心轴并与板分离。

公开(公告)号：US20100193705A1

公开(公告)日：2010-08-05

申请号：US12718178

申请日：2010-03-05

申请人： Brian Rasnow ,  Chuck Z. Li

发明人： Brian Rasnow ,  Chuck Z. Li

IPC分类号： G01N21/64

CPC分类号： G01N21/6454

摘要： An apparatus is provided that is capable of interleaving detection of fluorescence and luminescence signals emitted from a plurality of samples. The apparatus is suitable for analysis of samples containing single cells or tissues up to and including living organisms. It contains an optical assembly or “sandwich” for producing a spectrally pure and spatially disperse light source for illuminating the sample. The invention also provides a plurality of optical sandwiches that can be variously geometrically arranged and their intensities programmed to create spatially uniform illumination over a large sample. The invention further provides an apparatus having at least one of the optical sandwich and a detector system capable of interleaving detection of fluorescent and luminescent signals when a suitable sample is illuminated by the light source of the optical sandwich. Methods for preparing samples and using the sandwiches, arrays and apparatus, are further provided by this invention. A method for interleaving detection of fluorescent and luminescent signals emitted from a plurality of samples is disclosed.

摘要翻译： 提供一种能够交错检测从多个样品发射的荧光和发光信号的装置。 该装置适用于分析含有直至并包括活生物体的单个细胞或组织的样品。 它包含用于产生用于照亮样品的光谱纯的和空间分散的光源的光学组件或“夹心”。 本发明还提供了多个光学三明治，其可以在各种几何上布置，并且其强度被编程以在大样品上产生空间均匀的照明。 本发明还提供一种具有光学三明治和检测器系统中的至少一个的装置，当由光学三明治的光源照射合适的样品时，能够交错检测荧光和发光信号。 本发明还提供了制备样品和使用三明治，阵列和装置的方法。 公开了一种用于交叉检测从多个样本发射的荧光和发光信号的方法。