公开(公告)号：US20050089873A1

公开(公告)日：2005-04-28

申请号：US10690600

申请日：2003-10-23

Applicant: Dar-Fu Tai ,  Chung-Yin Lin ,  Tzong-Zeng Wu

Inventor： Dar-Fu Tai ,  Chung-Yin Lin ,  Tzong-Zeng Wu

IPC: G01N27/00 ,  G01N33/543 ,  C12Q1/68 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC classification number: G01N29/036 ,  G01N33/54353 ,  G01N2291/0257 ,  G01N2600/00 ,  Y10T436/141111

Abstract: Based on the direct formation of molecularly imprinted polymer on gold electrode, the present invention provides a peptide sensor for the detection of low-molecular-weight peptides. A new cross-linking monomer, (N-Acr-L-Cys-NHBn)2 is employed to attach the surface of the chip and to copolymerize with other monomers. Interestingly, N-benzylacrylamide participating both polymerization and recognition is carried out in an aqueous environment. Using quartz crystal microbalance detection, short peptides can be monitored by their interaction with plastic antibodies specific for the target peptides. The selectivity of molecularly imprinted polymer and the sensitivity of such artificial biosensors have collaborated to differentiate traces of oxytocin and vasopressin to the ng/ml scale.

Abstract translation: 基于在金电极上直接形成分子印迹聚合物，本发明提供了用于检测低分子量肽的肽传感器。 使用新的交联单体（N-Acr-L-Cys-NHBn）2 N以使芯片的表面附着并与其它单体共聚。 有趣的是，参与聚合和识别的N-苄基丙烯酰胺在水性环境中进行。 使用石英晶体微量天平检测，短肽可以通过与靶肽特异性的塑料抗体的相互作用来监测。 分子印迹聚合物的选择性和这种人造生物传感器的敏感性已经合作将催产素和加压素的痕量区分到ng / ml量表。

公开(公告)号：US20080226503A1

公开(公告)日：2008-09-18

申请号：US12109352

申请日：2008-04-25

Applicant: Dar-Fu Tai ,  Chung-Yin Lin ,  Tzong-Zeng Wu

Inventor： Dar-Fu Tai ,  Chung-Yin Lin ,  Tzong-Zeng Wu

IPC: G01N33/48

CPC classification number: G01N29/036 ,  G01N33/54353 ,  G01N33/54373 ,  G01N2291/0257 ,  G01N2600/00

Abstract: This invention presents a fast and effective method to detect macromolecules such as peptides. Using a multifunctional chiral monomer, it combines molecular imprinting polymerization technology with a quartz crystal microbalance for detection of peptides to the ng/ml scale.

Abstract translation: 本发明提供了快速有效的检测大分子如肽的方法。 使用多功能手性单体，它将分子印迹聚合技术与石英晶体微量天平结合，以将肽检测到ng / ml标度。