公开(公告)号：US20110070630A1

公开(公告)日：2011-03-24

申请号：US12866612

申请日：2009-03-26

申请人： Christian Gruber ,  Wolfgang Kroutil ,  Constance Voss ,  Jan Pfeffer ,  Uwe Dingerdissen

发明人： Christian Gruber ,  Wolfgang Kroutil ,  Constance Voss ,  Jan Pfeffer ,  Uwe Dingerdissen

IPC分类号： C12P41/00

CPC分类号： C12P41/002 ,  C12N9/0006 ,  C12P7/02

摘要： The invention relates to a process for enzymatic deracemization of enantiomer mixtures of secondary alcohols by a combination of oxidation and reduction reactions by means of stereoselective alcohol dehydrogenases and the cofactors thereof, wherein one enantiomer of an optically active secondary alcohol is in a formal sense selectively oxidized to the corresponding ketone, which is subsequently reduced selectively to the optical antipode, while the reduced form of the cofactor is provided for the reduction reaction by means of an additional enzyme, characterized in that two alcohol dehydrogenases with opposite stereoselectivity and different cofactor selectivity and the two corresponding, different cofactors are used for the oxidation and reduction reactions, and the oxidized and reduced cofactors are interconverted in a parallel enzymatic reaction with the additional enzyme, the direction of the deracemization toward one of the two enantiomers being controllable by the selection of the two alcohol dehydrogenases or using the selectivity difference of the additional enzyme for the two cofactors.

摘要翻译： 本发明涉及通过立体选择性醇脱氢酶及其辅因子通过氧化和还原反应的组合将仲醇的对映异构体混合物酶促脱水化的方法，其中光学活性仲醇的一种对映异构体具有形式上的选择性氧化 相应的酮，其随后被选择性地还原成光学对映体，而还原形式的辅因子被提供用于通过另外的酶的还原反应，其特征在于具有相反立体选择性和不同辅因子选择性的两种醇脱氢酶和 两个相应的不同的辅因子被用于氧化和还原反应，并且氧化的和还原的辅因子与另外的酶在平行的酶反应中相互转化，通过选择th可以控制两个对映异构体之一的旋转方向 使用两种醇脱氢酶或使用另外的酶对两种辅因子的选择性差异。