公开(公告)号：US06586196B1

公开(公告)日：2003-07-01

申请号：US09459982

申请日：1999-12-14

申请人： Irena Bronstein ,  Christopher Martin ,  Corinne Olesen ,  John Voyta ,  Yu-xin Yan

发明人： Irena Bronstein ,  Christopher Martin ,  Corinne Olesen ,  John Voyta ,  Yu-xin Yan

IPC分类号： C12Q142

CPC分类号： C12Q1/6897 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/66 ,  G01N2333/916 ,  G01N2333/924 ,  G01N2333/95

摘要： The present invention discloses multiple enzyme assays which measure the activity of at least one endogenous enzyme in a single aliquot and a method of measuring the activity of multiple enzymes in an aliquot of a cell extract, wherein at least one of the enzymes is an endogenous enzyme. In one embodiment of the invention the activity of a first enzyme is quantified by measuring the light signal produced by degradation of a first enzyme substrate by the first enzyme and the activity of the second enzyme is quantified by measuring the light signal produced by the degradation of a second substrate. In the method of the present invention, both quantifications are performed on the same aliquot of cell extract. Different embodiments of the present invention provide for the detection of more than one endogenous enzyme and for the detection of at least one reporter enzyme and at least one endogenous enzyme. The present invention also discloses kits for detecting the activity of multiple enzymes.

摘要翻译： 本发明公开了测量单一等分试样中至少一种内源性酶的活性的多种酶测定法和测量细胞提取物等分试样中多种酶的活性的方法，其中至少一种酶是内源性酶 。 在本发明的一个实施方案中，通过测量由第一酶降解第一酶底物产生的光信号来定量第一酶的活性，并且通过测量由第一酶降解产生的光信号来量化第二酶的活性 第二基板。 在本发明的方法中，两种定量在相同等分的细胞提取物上进行。 本发明的不同实施方案提供了多于一种内源性酶的检测和用于检测至少一种报道酶和至少一种内源性酶。 本发明还公开了用于检测多种酶的活性的试剂盒。