公开(公告)号：US10131933B2

公开(公告)日：2018-11-20

申请号：US14349737

申请日：2012-09-03

申请人： Gregory Stevens ,  Michael Krueger ,  Andrea Zgaga-Griesz ,  Gerald Urban ,  Dalice Silver

发明人： Gregory Stevens ,  Michael Krueger ,  Andrea Zgaga-Griesz ,  Gerald Urban ,  Dalice Silver

IPC分类号： C12Q1/66 ,  C12Q1/37 ,  C12N9/02

摘要： The present invention relates to materials and methods suitable for determining the presence or amount of Botulinum toxin (BoNT) in a test sample by means of a luminescence assay in which the substrate peptide is composed of: an amino acid sequence susceptible to proteolytic cleavage by BoNT, an amino acid sequence corresponding to a reporter domain encoding a fluorescent or bioluminescent polypeptide, and a tag suitable for attaching the substrate peptide to a suitable support, preferably by covalent bond.

公开(公告)号：US20140273039A1

公开(公告)日：2014-09-18

申请号：US14349737

申请日：2012-09-03

申请人： Gregory Stevens ,  Michael Krueger ,  Andrea Zgaga-Griesz ,  Gerald Urban ,  Dalice Silver

发明人： Gregory Stevens ,  Michael Krueger ,  Andrea Zgaga-Griesz ,  Gerald Urban ,  Dalice Silver

IPC分类号： C12Q1/66 ,  C12N9/02

CPC分类号： C12Q1/66 ,  C12N9/0069 ,  C12Q1/37 ,  G01N2333/33

摘要： The present invention relates to a method of determining Botulinum toxin (BoNT) based on a luminescence assay. The present application further relates to a peptide that is susceptible to proteolytic cleavage by BoNT which is suitable for that method.

摘要翻译： 本发明涉及一种基于发光测定法测定肉毒杆菌毒素（BoNT）的方法。 本申请还涉及适用于该方法的BoNT易于蛋白水解切割的肽。