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公开(公告)号：US07582461B2
公开(公告)日：2009-09-01
申请号：US10903529
申请日：2004-07-29
申请人： Elizabeth A. Werner , Tony A. Klink , Jane A. Beebe , David A. Lasky , Karen M. Kleman-Leyer , Richard Somberg
发明人： Elizabeth A. Werner , Tony A. Klink , Jane A. Beebe , David A. Lasky , Karen M. Kleman-Leyer , Richard Somberg
CPC分类号： C12Q1/485 , A61K38/00 , C07K7/06 , C07K7/08 , G01N2333/81 , G01N2333/9121
摘要： Compositions, methods, and kits for detecting and monitoring kinase or phosphatase activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase or phosphatase alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase or phosphatase activity are also described.
摘要翻译： 描述了用于检测和监测激酶或磷酸酶活性的组合物，方法和试剂盒。 组合物通常包括肽，可检测部分和蛋白酶切割位点。 通过激酶或磷酸酶改变肽改变肽的蛋白水解敏感性，导致组合物的可检测性质的变化。 还描述了用于确定激酶或磷酸酶活性的底物或调节剂的面板测定。
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公开(公告)号：US20120083427A1
公开(公告)日：2012-04-05
申请号：US13252679
申请日：2011-10-04
CPC分类号： C12Q1/42 , A61K38/00 , C07K7/06 , C07K7/08 , C12Q1/485 , G01N33/573 , G01N2333/81 , G01N2333/9121
摘要： Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.
摘要翻译： 描述了用于检测和监测激酶，磷酸酶和蛋白质翻译后修饰活性的组合物，方法和试剂盒。 组合物通常包括肽，可检测部分和蛋白酶切割位点。 通过激酶，磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性，导致组合物的可检测性质的改变。 还描述了用于确定激酶，磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。
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公开(公告)号：US07727752B2
公开(公告)日：2010-06-01
申请号：US11485957
申请日：2006-07-14
CPC分类号： C12Q1/42 , A61K38/00 , C07K7/06 , C07K7/08 , C12Q1/485 , G01N33/573 , G01N2333/81 , G01N2333/9121
摘要： Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.
摘要翻译： 描述了用于检测和监测激酶，磷酸酶和蛋白质翻译后修饰活性的组合物，方法和试剂盒。 组合物通常包括肽，可检测部分和蛋白酶切割位点。 通过激酶，磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性，导致组合物的可检测性质的变化。 还描述了用于确定激酶，磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。
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公开(公告)号：US20100240080A1
公开(公告)日：2010-09-23
申请号：US12728926
申请日：2010-03-22
CPC分类号： C12Q1/42 , A61K38/00 , C07K7/06 , C07K7/08 , C12Q1/485 , G01N33/573 , G01N2333/81 , G01N2333/9121
摘要： Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.
摘要翻译： 描述了用于检测和监测激酶，磷酸酶和蛋白质翻译后修饰活性的组合物，方法和试剂盒。 组合物通常包括肽，可检测部分和蛋白酶切割位点。 通过激酶，磷酸酶或其他蛋白质翻译后修饰来修饰肽改变了肽的蛋白水解敏感性，导致组合物的可检测性质的变化。 还描述了用于确定激酶，磷酸酶或其他蛋白质翻译后修饰活性的底物或调节剂的面板测定法。
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公开(公告)号：US07619059B2
公开(公告)日：2009-11-17
申请号：US10937042
申请日：2004-09-09
申请人： Kevin R. Kupcho , Kurt Vogel , Elizabeth A. Werner , Jane A. Beebe , Tony A. Klink , David A. Lasky , Karen M. Kleman-Leyer , Richard Somberg
发明人： Kevin R. Kupcho , Kurt Vogel , Elizabeth A. Werner , Jane A. Beebe , Tony A. Klink , David A. Lasky , Karen M. Kleman-Leyer , Richard Somberg
CPC分类号： C12Q1/42 , C12Q1/485 , G01N2440/14
摘要： Compositions, methods, and kits for detecting and monitoring post-translational modification activities, including kinase or phosphatase activities, are described. The compositions typically include a peptide, a first detectable moiety, a first binding member, and a protease cleavage site. Modification of a composition by a post-translational modification enzyme, such as a kinase or phosphatase, alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition when it is associated noncovalently with a probe composition that includes a second binding member and a second detectable moiety. Panel assays for determining substrates or modulators of enzymatic activities are also described.
摘要翻译： 描述了用于检测和监测翻译后修饰活性（包括激酶或磷酸酶活性）的组合物，方法和试剂盒。 组合物通常包括肽，第一可检测部分，第一结合成员和蛋白酶切割位点。 通过翻译后修饰酶（例如激酶或磷酸酶）修饰组合物改变了肽的蛋白水解敏感性，导致当组合物非共价地与探针组合物相关联时，组合物的可检测性质发生变化，所述探针组合物包括 第二结合成员和第二可检测部分。 还描述了用于确定酶活性的底物或调节剂的面板测定。
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