公开(公告)号：US06461831B1

公开(公告)日：2002-10-08

申请号：US09509203

申请日：2000-09-21

申请人： David Henry Small ,  Javier Saez-Valero ,  Gian Sberna

发明人： David Henry Small ,  Javier Saez-Valero ,  Gian Sberna

IPC分类号： C12Q146

CPC分类号： C12N9/18 ,  G01N33/6896 ,  G01N2333/916 ,  G01N2800/2821 ,  Y10S435/827

摘要： A method for the diagnosis of Alzheimer's disease (AD) in a patient, comprising the steps of: (1) providing a sample of an appropriate body fluid from said patient; (2) detecting the presence of acetylcholinesterase (AChE) with an altered glycosylation pattern in said sample. It has been established that approximately 75-95% of the AChE in the CSF of AD patients binds to Concanavalin (Con A) or wheat germ agglutinin (WGA) but with different specificity to each. Accordingly, in order to identify the glycosylation pattern of AChE in the sample, the binding to Con A is determined, then the binding to WGA is determined, and a ratio calculated. The ratio is characteristic of the glycosylation pattern. In alternative embodiment of the invention a monoclonal antibody specific for AChE with an altered glycosylation pattern is used to detect its presence.

摘要翻译： 一种用于诊断患者中的阿尔茨海默病（AD）的方法，包括以下步骤：（1）从所述患者提供适当体液的样品; （2）检测所述样品中糖基化模式改变的乙酰胆碱酯酶（AChE）的存在。 已经确定，AD患者CSF中约75-95％的AChE与伴刀豆球蛋白（Con A）或小麦胚芽凝集素（WGA）结合，但具有不同的特异性。 因此，为了鉴定样品中AChE的糖基化模式，确定与Con A的结合，然后测定与WGA的结合，并计算比例。 该比例是糖基化模式的特征。 在本发明的替代实施方案中，使用对具有改变的糖基化模式的AChE特异性的单克隆抗体来检测其存在。