公开(公告)号：US06403329B1

公开(公告)日：2002-06-11

申请号：US09548665

申请日：2000-04-13

申请人： Thaddeus John Novak ,  Herbert Samuel Aaron ,  Tracey Denise Biggs

发明人： Thaddeus John Novak ,  Herbert Samuel Aaron ,  Tracey Denise Biggs

IPC分类号： C12Q146

CPC分类号： C12Q1/46 ,  G01N31/22 ,  G01N33/52

摘要： Methods and apparatus are disclosed for screening chemical samples to detect the presence of chemical warfare agents, chemical warfare agent precursors and degradation products formed therefrom. Particular applications include detection of the presence of a cholinesterase inhibitor derived from alkyloxy methylphosphonic acids, methylphosphonic acid, and methylphosphonofluoridic acid.

摘要翻译： 公开了用于筛选化学样品以检测化学战剂，化学战剂前体和由其形成的降解产物的存在的方法和装置。 具体应用包括检测衍生自烷氧基甲基膦酸，甲基膦酸和甲基膦酰氟酸的胆碱酯酶抑制剂的存在。

公开(公告)号：US06764831B2

公开(公告)日：2004-07-20

申请号：US09444459

申请日：1999-11-22

申请人： Bruce M. Cameron, Sr. ,  Robert C. Allen

发明人： Bruce M. Cameron, Sr. ,  Robert C. Allen

IPC分类号： C12Q146

CPC分类号： G01N33/523 ,  A61K38/465 ,  C12Q1/46 ,  C12Q2334/30 ,  C12Q2334/70 ,  C12Y301/01007 ,  G01N33/9406 ,  G01N33/944 ,  G01N2800/52 ,  Y10S435/975

摘要： The present invention provides novel methods and diagnostic kits for the objective measurement of the severity of pain or stress being experienced by a patient with a disorder, diagnosis and treatment for patients suffering from painful disorders, and monitoring the effectiveness of different pain-treatment protocols. Pain-measuring methods comprise collecting a sample from a patient and determining the presence of a pain-associated marker in the sample. Methods for alleviating pain comprise administrating a dose of a therapeutically effective amount of a composition to the patient wherein the dose is determined by the presence of a pain-associated marker in a biological sample obtained from the patient. Compositions for alleviating pain comprise substances that are pain-associated markers or agents that interfere with pain-associated markers, and block or modulate the progression of pain perceived by the patient.

摘要翻译： 本发明提供了用于客观测量患有疼痛病症的患者的病症，诊断和治疗的患者经历的疼痛或压力的严重程度以及监测不同疼痛治疗方案的有效性的新型方法和诊断试剂盒。 疼痛测量方法包括从患者收集样品并确定样品中疼痛相关标志物的存在。 减轻疼痛的方法包括向患者施用治疗有效量的组合物的剂量，其中通过在从患者获得的生物样品中存在疼痛相关标志物来确定剂量。 用于缓解疼痛的组合物包括与疼痛相关的标志物或干扰疼痛相关标志物的试剂以及阻断或调节患者感觉到的疼痛进展的物质。

公开(公告)号：US06461831B1

公开(公告)日：2002-10-08

申请号：US09509203

申请日：2000-09-21

申请人： David Henry Small ,  Javier Saez-Valero ,  Gian Sberna

发明人： David Henry Small ,  Javier Saez-Valero ,  Gian Sberna

IPC分类号： C12Q146

CPC分类号： C12N9/18 ,  G01N33/6896 ,  G01N2333/916 ,  G01N2800/2821 ,  Y10S435/827

摘要： A method for the diagnosis of Alzheimer's disease (AD) in a patient, comprising the steps of: (1) providing a sample of an appropriate body fluid from said patient; (2) detecting the presence of acetylcholinesterase (AChE) with an altered glycosylation pattern in said sample. It has been established that approximately 75-95% of the AChE in the CSF of AD patients binds to Concanavalin (Con A) or wheat germ agglutinin (WGA) but with different specificity to each. Accordingly, in order to identify the glycosylation pattern of AChE in the sample, the binding to Con A is determined, then the binding to WGA is determined, and a ratio calculated. The ratio is characteristic of the glycosylation pattern. In alternative embodiment of the invention a monoclonal antibody specific for AChE with an altered glycosylation pattern is used to detect its presence.

摘要翻译： 一种用于诊断患者中的阿尔茨海默病（AD）的方法，包括以下步骤：（1）从所述患者提供适当体液的样品; （2）检测所述样品中糖基化模式改变的乙酰胆碱酯酶（AChE）的存在。 已经确定，AD患者CSF中约75-95％的AChE与伴刀豆球蛋白（Con A）或小麦胚芽凝集素（WGA）结合，但具有不同的特异性。 因此，为了鉴定样品中AChE的糖基化模式，确定与Con A的结合，然后测定与WGA的结合，并计算比例。 该比例是糖基化模式的特征。 在本发明的替代实施方案中，使用对具有改变的糖基化模式的AChE特异性的单克隆抗体来检测其存在。

公开(公告)号：US06291200B1

公开(公告)日：2001-09-18

申请号：US09441596

申请日：1999-11-17

申请人： Keith E. LeJeune ,  Alan J. Russell

发明人： Keith E. LeJeune ,  Alan J. Russell

IPC分类号： C12Q146

CPC分类号： C12Q1/58 ,  C12Q1/34 ,  C12Q1/46 ,  G01N33/521

摘要： A sensor for detecting the presence of at least one analyte includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a product compound or (ii) be inhibited by the analyte in the presence of a substrate compound. The sensor also includes at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the analyte and the enzyme. Each of the enzyme and the indicator compound are incorporated within a single polymer.

摘要翻译： 用于检测至少一种分析物的存在的传感器包括至少一种酶，其被选择为（i）催化分析物的反应以将分析物化学转化成产物化合物或（ii）被分析物在 底物化合物的存在。 传感器还包括至少一种指示剂化合物，其被选择用于由于分析物和酶的相互作用而产生可测量的状态变化。 酶和指示剂化合物中的每一种都掺入单一聚合物中。

公开(公告)号：US06746850B2

公开(公告)日：2004-06-08

申请号：US09848370

申请日：2001-05-04

申请人： Shawn R. Feaster ,  Richard K. Gordon ,  Bhupendra P. Doctor

发明人： Shawn R. Feaster ,  Richard K. Gordon ,  Bhupendra P. Doctor

IPC分类号： C12Q146

CPC分类号： C12Q1/005 ,  C12Q1/46 ,  G01N33/557 ,  G01N33/567

摘要： An assay for detecting, measuring, or monitoring the activity or concentration of at least two proteins that have similar or overlapping properties is disclosed. The assay comprises first determining the sensitivity coefficients of the substrates for each of the proteins in which the concentrations are to be determined. This method may be used for detecting, measuring, or monitoring the activity and concentration of AChE, BChE, or both in a test sample which test sample may be whole and unprocessed blood or tissue. Also disclosed are methods of using the assay to detect a subject's exposure to an agent which affects cholinesterase, determine the efficacy or progress of a treatment, determine the amount of protection provided against exposure to an agent which affects cholinesterase, or both, screen a subject for having a drug sensitivity or a particular disease, detect a change in red blood cell count of a subject, determine whether a candidate compound affects cholinesterase. Also disclosed are devices and kits for detecting, measuring, or monitoring the activities and concentrations of AChE, BChE, or both.

公开(公告)号：US06673565B2

公开(公告)日：2004-01-06

申请号：US09952112

申请日：2001-09-14

申请人： Keith E. LeJeune ,  Alan J. Russel

发明人： Keith E. LeJeune ,  Alan J. Russel

IPC分类号： C12Q146

CPC分类号： C12Q1/58 ,  C12Q1/34 ,  C12Q1/46 ,  G01N33/521

摘要： A sensor for detecting an analyte enzyme includes at least one substrate compound and at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the substrate and at least one target or analyte enzyme. Each of the indicator(s) and substrate(s) are incorporated within a single polymer.

摘要翻译： 用于检测分析物酶的传感器包括至少一种底物化合物和至少一种指示剂化合物，所述至少一种指示剂化合物是由于底物和至少一种靶标或分析物酶的相互作用而产生可测量的状态变化。 指示剂和底物中的每一个都结合在单一聚合物中。

公开(公告)号：US06406876B1

公开(公告)日：2002-06-18

申请号：US09558511

申请日：2000-04-26

申请人： Richard K. Gordon ,  Bhupendra P. Doctor ,  Ashima Saxena ,  Shawn R. Feaster ,  Donald Maxwell ,  Michelle Ross ,  David Lenz ,  Keith LeJeune ,  Alan Russell

发明人： Richard K. Gordon ,  Bhupendra P. Doctor ,  Ashima Saxena ,  Shawn R. Feaster ,  Donald Maxwell ,  Michelle Ross ,  David Lenz ,  Keith LeJeune ,  Alan Russell

IPC分类号： C12Q146

CPC分类号： C12Q1/34 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/46 ,  Y10S435/963 ,  Y10S435/97

摘要： Methods, compositions and materials useful in the detection of organophosphorous and organosulfur compounds are disclosed. In particular, biosensors wherein a porous or a non-porous support having an enzyme immobilized upon or within are disclosed. The biosensors exhibit enzymatic stability at extreme temperatures and/or denaturing conditions, and similar kinetic characteristics of the soluble form of the enzymes utilized. The enzyme does not leach from the porous or non-porous support and the material retains enzymatic activity after prolonged storage. Differential biosensors are also disclosed.

摘要翻译： 公开了用于检测有机磷和有机硫化合物的方法，组合物和材料。 特别地，公开了其中固定有或内部固定有酶的多孔或无孔载体的生物传感器。 生物传感器在极端温度和/或变性条件下表现出酶稳定性，以及所用酶的可溶形式的类似动力学特征。 该酶不会从多孔或非多孔载体中浸出，并且材料在长时间储存​​后保留酶活性。 还公开了差示生物传感器。