Poxvirus insertion/expression vector
    3.
    发明授权
    Poxvirus insertion/expression vector 失效
    位点特异性RNA切割

    公开(公告)号:US5443964A

    公开(公告)日:1995-08-22

    申请号:US84406

    申请日:1987-08-10

    CPC分类号: C12N15/86 C12N2710/24143

    摘要: The invention comprises a plasmid vector, utilizing genetic material that causes high levels of expression of poxvirus genes, and the method of using this genetic material. One preferred embodiment comprises an upstream cis-acting element of a poxvirus gene encoding the major protein component of the poxvirus A-type inclusion. A second preferred embodiment of the invention comprises a downstream cis-acting element of a poxvirus gene encoding the major protein component of the poxvirus A-type inclusion. These elements are inserted upstream and downstream respectively, of selected cloned genes in the plasmid vector to obtain very high levels of expression of the cloned gene.

    摘要翻译: 本发明包括使用导致痘病毒基因高水平表达的遗传物质的质粒载体,以及使用该遗传物质的方法。 一个优选的实施方案包括编码痘病毒A型包涵体的主要蛋白质成分的痘病毒基因的上游顺式作用元件。 本发明的第二个优选实施方案包括编码痘病毒A型包合物的主要蛋白质组分的痘病毒基因的下游顺式作用元件。 这些元件分别插入质粒载体中所选克隆基因的上游和下游,以获得克隆基因的非常高水平的表达。