公开(公告)号：US20140274953A1

公开(公告)日：2014-09-18

申请号：US13827170

申请日：2013-03-14

申请人： Woo Lee ,  Jenny Zilberberg ,  David Samuel Siegel ,  Peter Tolias ,  Hongjun Wang ,  Wenting Zhang

发明人： Woo Lee ,  Jenny Zilberberg ,  David Samuel Siegel ,  Peter Tolias ,  Hongjun Wang ,  Wenting Zhang

IPC分类号： G01N33/50

CPC分类号： C12N5/0694 ,  G01N33/5091 ,  G01N33/574 ,  G01N2500/10 ,  G01N2510/00 ,  G01N2800/52 ,  G01N2800/7028

摘要： The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.

摘要翻译： 所述发明提供了包含在微流体装置中的离体动态多发性骨髓瘤（MM）癌症小生境。 动态MM癌症利基包括（a）含有动态离体骨髓（BM）利基的三维组织构建体，其包含自体组织成粘性多细胞层的活性成骨细胞的矿化骨样组织和细胞外基质 由可行的粘附成骨细胞分泌; 和营养物质和溶解气体分子动态灌注的微环境; 和（b）从包含单核细胞和多发性骨髓瘤细胞的生物壳组合物接种的人骨髓瘤细胞。 人骨髓瘤细胞与BM细菌的成骨细胞接触，并且通过MM癌症细菌保持人骨髓瘤细胞的存活力。