公开(公告)号：US5132221A

公开(公告)日：1992-07-21

申请号：US350662

申请日：1989-05-10

申请人： Thomas W. Ward ,  Debby F. Bruhn ,  Deborah K. Bulmer

发明人： Thomas W. Ward ,  Debby F. Bruhn ,  Deborah K. Bulmer

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N2795/14221 ,  C12N2795/14232

摘要： A bacteriophage identified as .phi.Ac1 capable of infecting acidophilic heterotropic bacteria (such as Acidiphilium sp.) and processes for genetically engineering acidophilic bacteria for biomining or sulfur removal from coal are disclosed. The bacteriophage is capable of growth in cells existing at pH at or below 3.0. Lytic forms of the phage introduced into areas experiencing acid drainage kill the bacteria causing such drainage. Lysogenic forms of the phase having genes for selective removal of metallic or nonmetallic elements can be introduced into acidophilic bacteria to effect removal of the desired element form ore or coal.

摘要翻译： 公开了鉴定为能够感染嗜酸异质细菌（例如嗜酸菌属）的phi Ac1的噬菌体和用于遗传工程用于从煤生物或硫去除的嗜酸细菌的方法。 噬菌体能够在pH在3.0以下存在的细胞中生长。 引入到经过酸性排泄的区域的噬菌体的裂纹形式杀死引起这种排泄的细菌。 可以将具有用于选择性去除金属或非金属元素的基因的相的溶原性形式引入嗜酸细菌中，以除去所需要的元素形式的矿石或煤。

公开(公告)号：US06210937B1

公开(公告)日：2001-04-03

申请号：US09064693

申请日：1998-04-22

申请人： Thomas E. Ward ,  Carolyn S. Watkins ,  Deborah K. Bulmer ,  Bruce F. Johnson ,  Mohan Amaratunga

发明人： Thomas E. Ward ,  Carolyn S. Watkins ,  Deborah K. Bulmer ,  Bruce F. Johnson ,  Mohan Amaratunga

IPC分类号： C12P742

CPC分类号： C12P7/42 ,  C12N15/52

摘要： The cloning and expression of genes in the common aromatic pathway of E. coli are described. A compound for which chorismate, the final product of the common aromatic pathway, is an anabolic intermediate can be produced by cloning and expressing selected genes of the common aromatic pathway and the genes coding for enzymes necessary to convert chorismate to the selected compound. Plasmids carrying selected genes of the common aromatic pathway are also described.

摘要翻译： 描述了大肠杆菌共同芳香途径中基因的克隆和表达。 可以通过克隆和表达常见芳香途径的选定基因和编码将选择性化合物转化佐丹酸所必需的酶的基因来制备共振芳香途径的最终产物是合成代谢中间体的化合物。 还描述了携带选定的共同芳香途径基因的质粒。