公开(公告)号：US20090197335A1

公开(公告)日：2009-08-06

申请号：US12364409

申请日：2009-02-02

Applicant: Deepak Kumar Agrawal ,  Warren Jackson Pledger ,  Jonathan A. Kluft ,  Asha Agrawal

Inventor： Deepak Kumar Agrawal ,  Warren Jackson Pledger ,  Jonathan A. Kluft ,  Asha Agrawal

IPC: C12N15/01 ,  C12N5/00

CPC classification number: C12N15/87

Abstract: The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.

Abstract translation: 本发明涉及电穿孔缓冲液，其允许在施加电流期间增强细胞的转染效率和细胞活力。 本发明的缓冲液提供目标颗粒到细胞中的最大转移，同时保持细胞群的健康和生长潜力。 本发明的组合物包含大约生理离子强度和pH的电穿孔缓冲液，并且添加有血清或纯化的蛋白质，例如血清白蛋白。 本发明适用于任何细胞类型。 本发明还涉及使用本发明的电穿孔缓冲液的电穿孔方法。

公开(公告)号：US08551780B2

公开(公告)日：2013-10-08

申请号：US12364409

申请日：2009-02-02

Applicant: Deepak Kumar Agrawal ,  Warren Jackson Pledger ,  Jonathan A. Kluft ,  Asha Agrawal

Inventor： Deepak Kumar Agrawal ,  Warren Jackson Pledger ,  Jonathan A. Kluft ,  Asha Agrawal

IPC: C07K14/435

CPC classification number: C12N15/87

Abstract: The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.

Abstract translation: 本发明涉及电穿孔缓冲液，其允许在施加电流期间增强细胞的转染效率和细胞活力。 本发明的缓冲液提供目标颗粒到细胞中的最大转移，同时保持细胞群的健康和生长潜力。 本发明的组合物包含大约生理离子强度和pH的电穿孔缓冲液，并且添加有血清或纯化的蛋白质，例如血清白蛋白。 本发明适用于任何细胞类型。 本发明还涉及使用本发明的电穿孔缓冲液的电穿孔方法。