公开(公告)号：US06251406B1

公开(公告)日：2001-06-26

申请号：US09413807

申请日：1999-10-07

申请人： Denise N. Haefliger ,  Jean-Pierre Kraehenbuhl

发明人： Denise N. Haefliger ,  Jean-Pierre Kraehenbuhl

IPC分类号： A61K39112

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/51 ,  C07K2319/00 ,  C12N15/74 ,  C12N2710/20022 ,  C12N2710/20023 ,  Y02A50/476 ,  Y02A50/484 ,  Y10S977/804 ,  Y10S977/904 ,  Y10S977/917

摘要： This application relates to the use of attenuated prokaryotic miccrooganism strains (such as Salmonella) expressing nucleic acid encoding HPV proteins as vaccines against HPV infection and he associated increased risk of cancer. In particular, the work shows that it is possible to assemble VLPs in a prokaryotic organism and that nasal immunization of mice with the strains HPV-specific conformationally dependent and neutralizing antibodies in serum and genital secretions. The experiments described herein show that it is also possible to assemble chimeric VLPs of a HPV including a fusion partner and that tumor protection can be induced.

摘要翻译： 本申请涉及使用表达编码HPV蛋白的核酸的减毒原核微生态菌株（如沙门氏菌）作为HPV感染疫苗，并且与癌症风险增加有关。 特别是，这项工作表明，有可能在原核生物体中组装VLP，并且可以在血清和生殖道分泌物中对具有HPV特异性构象依赖性和中和抗体的菌株进行鼻腔免疫。 本文描述的实验表明，还可以组装包含融合伴侣的HPV的嵌合VLP并且可以诱导肿瘤保护。