公开(公告)号：US20080280318A1

公开(公告)日：2008-11-13

申请号：US11587123

申请日：2005-04-22

申请人： Elise Balse ,  Luc-Henri Te ssier ,  Celine Fuchs ,  Valerie Forster ,  Jose-Alain Sahel ,  Serge Picaud

发明人： Elise Balse ,  Luc-Henri Te ssier ,  Celine Fuchs ,  Valerie Forster ,  Jose-Alain Sahel ,  Serge Picaud

IPC分类号： C12Q1/02 ,  C12N5/02 ,  C12N5/06 ,  C12N5/08

CPC分类号： C12N5/062 ,  C12N2502/08 ,  C12N2503/02 ,  C12N2509/00

摘要： The present invention provides a process and kit for isolating cone photoreceptor cells from retinal tissue with a purity level of at least 80%, typically of about 90%. The isolation process uses a PNA-panning procedure conducted on dissociated retinal tissue. The present invention also provides a culture medium enabling the in vitro survival and development of such isolated cone cells. The means of the invention are applicable to adult mammalian cone cells, and more particularly to adult human cone cells. They have the advantage of being applicable to pathologic or otherwise altered cone cells, and thus give access to the screening of compounds capable of showing neuroprotective activity on adult cone cells.

摘要翻译： 本发明提供了用于从视网膜组织分离锥形感光细胞的方法和试剂盒，其纯度至少为80％，通常为约90％。 分离过程使用对解离的视网膜组织进行的PNA平移过程。 本发明还提供能够使这种分离的锥细胞的体外存活和发育的培养基。 本发明的方法适用于成年哺乳动物锥形细胞，更具体地涉及成年人锥形细胞。 它们具有适用于病理或其它改变的锥形细胞的优点，并且因此使得能够筛选能够对成体锥细胞显示神经保护活性的化合物进行筛选。