公开(公告)号：US12071627B2

公开(公告)日：2024-08-27

申请号：US18458752

申请日：2023-08-30

Applicant: Akouos, Inc.

Inventor： Emmanuel John Simons ,  Ellen Reisinger ,  Sebastian Kügler ,  Hanan Al-Moyed

IPC: C12N15/86 ,  A01K67/0276 ,  A61K38/17 ,  A61K48/00 ,  A61P27/16 ,  C07K14/47 ,  C12N5/0793 ,  C12N9/16 ,  C12N15/52 ,  C12N15/65 ,  C12N15/90

CPC classification number: C12N15/52 ,  A01K67/0276 ,  A61K38/1709 ,  A61K48/0075 ,  A61P27/16 ,  C07K14/47 ,  C12N5/062 ,  C12N9/16 ,  C12N15/65 ,  C12N15/86 ,  C12N15/902 ,  A01K2217/075 ,  A01K2227/103 ,  A01K2267/03 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2830/008 ,  C12Y301/03001

Abstract: Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject.

公开(公告)号：US20240254439A1

公开(公告)日：2024-08-01

申请号：US18417028

申请日：2024-01-19

Applicant: Korea University Research and Business Foundation

Inventor： Yong Taek JEONG ,  Mingyeong LEE

IPC: C12N5/0793

CPC classification number: C12N5/062 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2503/00 ,  C12N2506/097 ,  C12N2513/00

Abstract: A medium composition for inducing differentiation of taste bud organoids derived from the anterior tongue epithelium, a method for producing taste bud organoids, a taste bud organoid derived from the anterior tongue epithelium, and a method for screening a tastant using the same, wherein the medium composition for inducing differentiation of taste bud organoids derived from the anterior tongue epithelium and the method for producing taste bud organoids using the same may efficiently form the taste bud organoids from tongue epithelial tissue and may control their differentiation into specific types of taste cells. In addition, the taste bud organoids derived from the anterior tongue epithelium formed by the method may maximize the expression of markers for various taste buds and cells that make up taste buds to express salty taste detection proteins, so that they may be used in various fields such as cell sensors and tastant screening.

公开(公告)号：US20240228931A9

公开(公告)日：2024-07-11

申请号：US17769549

申请日：2020-10-12

Applicant: Wisconsin Alumni Research Foundation

Inventor： Zhenqiang Ma ,  Inkyu Lee ,  Juhwan Lee ,  Michael Phillips ,  David Gamm ,  Shaoqin Gong

IPC: C12M1/12 ,  C12N5/0793

CPC classification number: C12M25/14 ,  C12N5/062 ,  C12N2533/30

Abstract: Photoreceptor scaffolds that can be used for transplantation of organized photoreceptor tissue, with or without retinal pigment epithelial cells, which may improve grafted cell survival, integration, and functional visual rescue are disclosed herein. The scaffolds include a cell support layer having at least one honeycomb-shaped reservoir fluidly connected to a plurality of through-holes and at least one cell in the at least one honeycomb-shaped reservoir.

公开(公告)号：US11993777B2

公开(公告)日：2024-05-28

申请号：US18458744

申请日：2023-08-30

Applicant: Akouos, Inc.

Inventor： Emmanuel John Simons ,  Ellen Reisinger ,  Sebastian Kügler ,  Hanan Al-Moyed

IPC: C12N15/52 ,  A01K67/027 ,  A01K67/0276 ,  A61K38/17 ,  A61K48/00 ,  A61P27/16 ,  C07K14/47 ,  C12N5/0793 ,  C12N9/16 ,  C12N15/65 ,  C12N15/86 ,  C12N15/90

CPC classification number: C12N15/52 ,  A01K67/0276 ,  A61K38/1709 ,  A61K48/0075 ,  A61P27/16 ,  C07K14/47 ,  C12N5/062 ,  C12N9/16 ,  C12N15/65 ,  C12N15/86 ,  C12N15/902 ,  A01K2217/075 ,  A01K2227/103 ,  A01K2267/03 ,  C12N2310/20 ,  C12N2750/14143 ,  C12N2830/008 ,  C12Y301/03001

Abstract: Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject.

公开(公告)号：US20240060040A1

公开(公告)日：2024-02-22

申请号：US18032051

申请日：2021-10-14

Applicant: Ear Science Institute Australia

Inventor： Yee Man Elaine Wong

IPC: C12N5/0793 ,  A61K35/30 ,  G01N33/50

CPC classification number: C12N5/062 ,  A61K35/30 ,  G01N33/5058 ,  C12N2500/84 ,  C12N2501/15 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2506/45 ,  C12N2503/02

Abstract: The present invention relates to methods and compositions for producing differentiated otic cells. In particular, the invention relates to methods and compositions for the production of inner ear cells from pluripotent stem cells. The present invention also relates to methods of treating sensorineural hearing loss.

公开(公告)号：US20230407315A1

公开(公告)日：2023-12-21

申请号：US18458744

申请日：2023-08-30

Applicant: Akouos, Inc.

Inventor： Emmanuel John Simons ,  Ellen Reisinger ,  Sebastian Kügler ,  Hanan Al-Moyed

IPC: C12N15/52 ,  A01K67/027 ,  C12N15/65 ,  A61K48/00 ,  C07K14/47 ,  A61P27/16 ,  A61K38/17 ,  C12N5/0793 ,  C12N9/16 ,  C12N15/86 ,  C12N15/90

CPC classification number: C12N15/52 ,  A01K67/0276 ,  C12N15/65 ,  A61K48/0075 ,  C07K14/47 ,  A61P27/16 ,  A61K38/1709 ,  C12N5/062 ,  C12N9/16 ,  C12N15/86 ,  C12N15/902 ,  C12N2750/14143 ,  C12N2310/20 ,  C12N2830/008 ,  A01K2267/03 ,  A01K2217/075 ,  A01K2227/103 ,  C12Y301/03001

Abstract: Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject.

公开(公告)号：US11821893B2

公开(公告)日：2023-11-21

申请号：US17182528

申请日：2021-02-23

Applicant: University of Maryland, Baltimore County ,  UNIVERSITY OF MARYLAND, BALTIMORE

Inventor： Erin Lavik ,  Steve Bernstein ,  Adam Day ,  Bryan Ibarra

IPC: G09B23/30 ,  B41M3/06 ,  B33Y80/00 ,  G01N33/50 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0793

CPC classification number: G01N33/5008 ,  B33Y80/00 ,  B41M3/06 ,  C12N5/0062 ,  C12N5/062 ,  C12N5/0619 ,  C12N5/0679 ,  G09B23/30 ,  C12N2513/00 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2535/00

Abstract: A process of simply, cheaply, and reproducibly creating complex tissue models using screen printing and the tissue model prepared using the screen printing process. These models are amenable to high throughput screening. They will allow the study of components of disease progression and can be used for screening therapies.

公开(公告)号：US20230358770A1

公开(公告)日：2023-11-09

申请号：US18245992

申请日：2021-09-22

Applicant: Paul Feinstein ,  Charlotte D'Hulst

Inventor： Paul Feinstein ,  Charlotte D'Hulst ,  Elke Giets ,  Masayo Omura ,  Christopher Lemetre

IPC: G01N33/94 ,  C12N5/0793 ,  G01N33/50 ,  A01K67/027 ,  C07K14/705 ,  C12N15/86

CPC classification number: G01N33/9406 ,  C12N5/062 ,  G01N33/5091 ,  A01K67/0275 ,  C07K14/705 ,  C12N15/86 ,  G01N33/9413 ,  G01N2800/28 ,  G01N2333/70571 ,  C12N2740/15043 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0393 ,  G01N2800/14

Abstract: Provided herein are biosensors and methods for detecting one or more odorants associated with the levels, or a change in the levels, of one or more neurotransmitters in the central nervous system of a subject. In embodiments, provided are biosensors that comprise one or more populations of olfactory neurons, or cilia derived therefrom, wherein each population preferentially expresses a specific odorant receptor (OR). Also provided are biosensors comprising a cell or a population of cells engineered to express certain ORs; biosensors comprising certain isolated ORs; transgenic animals and tissues derived therefrom that preferentially express certain ORs; isolated cells or populations of cells engineered to express certain ORs; expression constructs for the preferential expression of certain ORs; and methods of using the biosensors, transgenic animals, tissues, cells, population of cells, and expression constructs disclosed herein.

公开(公告)号：US20230272339A1

公开(公告)日：2023-08-31

申请号：US18310048

申请日：2023-05-01

Applicant: The Johns Hopkins University

Inventor： Mandeep SINGH ,  Seth Blackshaw ,  Robert Johnston ,  Jiang Qian ,  Ying Liu

IPC: C12N5/0793

CPC classification number: C12N5/062 ,  C12N2513/00 ,  C12N2509/00

Abstract: The present disclosure provides methods for generating retinal cell clusters for use in cellular component transfer therapy, retinal cell clusters generated by such methods, and compositions comprising such retinal cell clusters. The present disclosure also provides uses of the retinal cell clusters and compositions comprising thereof for preventing and/or treating inherited retinal degenerative diseases.

公开(公告)号：US20230212509A1

公开(公告)日：2023-07-06

申请号：US18000008

申请日：2021-05-28

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  The United States of America, as represented by the Secretary, Department of Health and Human Servic

Inventor： Andrew DIAS ,  Erich BERNDT ,  Lucas CHASE ,  Juan AMARAL ,  Arvydas MAMINISHKIS ,  Kapil BHARTI

IPC: C12N5/0793 ,  C12N5/079 ,  A61L27/36 ,  C12N5/00 ,  A61L27/18 ,  A61L27/56 ,  A61L27/58 ,  A61L27/20 ,  A61L27/22 ,  A61L27/54

CPC classification number: C12N5/062 ,  A61L27/18 ,  A61L27/20 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L27/227 ,  A61L27/3641 ,  C12N5/0056 ,  C12N5/0621 ,  A61L2430/16 ,  C12N2533/40 ,  C12N2533/50 ,  C12N2533/90

Abstract: Provided herein are methods of producing a distinct bilayer culture of retinal epithelial cells (RPE) with photoreceptor cells and/or photoreceptor precursor cells (PR/PRP). Further provided herein is a therapy comprising transplantation of the RPE and PR/PRP bilayer as well as methods for testing candidate drugs using the bilayer.