公开(公告)号：US6136172A

公开(公告)日：2000-10-24

申请号：US470024

申请日：1999-12-22

申请人： John A. Renfrew ,  Eric Steinbach ,  Stuart MacMillan

发明人： John A. Renfrew ,  Eric Steinbach ,  Stuart MacMillan

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704 ,  G01N27/44743

摘要： Electrophoresis gels are formed using a gel-forming insert having a beveled edge which results in loading sites having a beveled bottom surface. The gel forming insert can have a continuous beveled edge across the entire width of the gel, in which case a special loading insert is used which matches the bevel of the gel. Alternatively, the gel forming insert may be formed with a plurality of fingers with beveled ends, each finger defining a well in the gel. In one form of the gel-forming insert, the fingers are formed from a soft, flexible polymer such as silicone applied on a rigid support.

公开(公告)号：US6110344A

公开(公告)日：2000-08-29

申请号：US885531

申请日：1997-06-30

申请人： John A. Renfrew ,  Eric Steinbach ,  Stuart MacMillan

发明人： John A. Renfrew ,  Eric Steinbach ,  Stuart MacMillan

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704

摘要： Electrophoresis gels are formed using a gel-forming insert having a beveled edge which results in loading sites having a beveled bottom surface. The gel forming insert can have a continuous beveled edge across the entire width of the gel, in which case a special loading insert is used which matches the bevel of the gel. Alternatively, the gel forming insert may be formed with a plurality of fingers with beveled ends, each finger defining a well in the gel. In one form of the gel-forming insert, the fingers are formed from a soft, flexible polymer such as silicone applied on a rigid support.

摘要翻译： 使用具有倾斜边缘的凝胶形成插入物形成电泳凝胶，其导致具有斜面底表面的装载位置。 凝胶形成插入件可以在凝胶的整个宽度上具有连续的斜边，在这种情况下，使用与凝胶的斜面相匹配的特殊加载插入物。 或者，凝胶形成插入物可以形成有多个具有倾斜端部的指状物，每个手指在凝胶中限定孔。 在凝胶形成插入物的一种形式中，指状物由柔软的柔性聚合物（例如施加在刚性支撑体上的硅树脂）形成。

公开(公告)号：US6007689A

公开(公告)日：1999-12-28

申请号：US836268

申请日：1997-05-01

申请人： John A. Renfrew ,  Eric Steinbach ,  John K. Stevens ,  Henryk Zaleski

发明人： John A. Renfrew ,  Eric Steinbach ,  John K. Stevens ,  Henryk Zaleski

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44704

摘要： An apparatus for the rapid preparation of electrophoresis gels comprises: (a) a housing; (b) a support fixture removably disposed within the housing and adapted to receive a gel holder having an internal gel compartment, the support fixture being optionally adapted to permit filling of the gel holder within the housing; (c) an optional injection system, which is connectible to a reservoir for holding a polymerizable solution; (d) an optional solution injection connector adapted to couple the injection system to a gel holder placed within the filling fixture, (e) an optional controller for the injection system, which causes the injection system to inject polymerizable solution from the reservoir into the gel compartment; and (f) a radiation source disposed within the housing in a location effective to irradiate polymierizable solution within the gel compartment of a gel holder in the support fixture. The support fixture may be mounted on a drawer which is slidable between a position inside the housing, and a position outside the housing to permit easy placement of a gel holder into the support fixture.

摘要翻译： PCT No.PCT / US95 / 13955 Sec。 371日期1997年5月1日 102（e）日期1997年5月1日PCT提交1995年10月31日PCT公布。 出版物WO96 / 日期：1996年5月9日。一种用于快速制备电泳凝胶的设备包括：（a）壳体; （b）可移除地设置在所述壳体内并适于接收具有内部凝胶隔室的凝胶保持器的支撑固定件，所述支撑固定件可选地适于允许在所述壳体内填充所述凝胶保持器; （c）可选的注射系统，其可连接到储存器以保持可聚合溶液; （d）可选的溶液注入连接器，其适于将注射系统耦合到置于填充夹具内的凝胶保持器，（e）用于注射系统的可选控制器，其使注射系统将可聚合溶液从储存器注入凝胶 隔间 以及（f）辐射源，其设置在所述壳体内的有效照射所述支撑夹具中的凝胶保持器的凝胶室内的可聚合溶液的位置。 支撑固定件可以安装在可在壳体内的位置和壳体外部的位置之间滑动的抽屉上，以允许将凝胶保持器容易地放置到支撑夹具中。