公开(公告)号：US06255047B1

公开(公告)日：2001-07-03

申请号：US09514827

申请日：2000-02-28

申请人： Kristen Toblesky ,  Douglas Kang ,  Alireza Ebrahim ,  Eric Vanderslice

发明人： Kristen Toblesky ,  Douglas Kang ,  Alireza Ebrahim ,  Eric Vanderslice

IPC分类号： C12Q100

CPC分类号： C12Q1/34 ,  G01N33/90 ,  G01N2333/79 ,  G01N2333/924

摘要： References, i.e., solutions of known concentration for use as controls, calibrators, or standards, for assays of carbohydrate-deficient transferring in bodily fluids are obtained from units of bodily fluids from normal healthy individuals by digesting the transferring in these units with exogenous neuraminidase to achieve concentrated CDT solutions, which are then added in preselected proportions to a base matrix to achieve target concentrations.

摘要翻译： 参考文献，即用作对照，校准物或标准品的已知浓度的溶液用于体液中碳水化合物缺乏转移的测定，从来自正常健康个体的体液的单位通过用外源性神经氨酸酶消化在这些单位中转移获得 实现浓缩的CDT溶液，然后将它们以预选的比例加入到碱性基质中以达到目标浓度。

公开(公告)号：US06548646B1

公开(公告)日：2003-04-15

申请号：US09938409

申请日：2001-08-23

申请人： Alireza Ebrahim ,  Jayshree Parikh ,  Warren Eric Vanderslice

发明人： Alireza Ebrahim ,  Jayshree Parikh ,  Warren Eric Vanderslice

IPC分类号： A61K3816

CPC分类号： G01N33/6893 ,  G01N2333/4737

摘要： Low-concentration reference controls for C-reactive protein assays are prepared from higher-level starting materials by simple filtration using silica-type filter media formerly used for removing lipids. This process avoids the need for antibodies to capture the protein and resuspend it. Surprisingly, the removal of CRP by the silica-type filter media is selective toward CRP, having little or negligible effect on other proteins in the starting matrix, and accordingly little or negligible change in the total protein content.

摘要翻译： 通过使用以前用于除去脂质的二氧化硅型过滤介质的简单过滤，从较高级别的起始原料制备C-反应蛋白测定的低浓度参考对照。 该过程避免了抗体捕获蛋白质并重新悬浮的需要。 令人惊奇的是，通过二氧化硅型过滤介质去除CRP对CRP是选择性的，对起始基质中的其它蛋白质的影响很小或可忽略不计，因此总蛋白质含量的变化很小或可忽略不计。