公开(公告)号：US09150926B2

公开(公告)日：2015-10-06

申请号：US12961298

申请日：2010-12-06

申请人： Electron Kebebew ,  Erin Patterson

发明人： Electron Kebebew ,  Erin Patterson

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N5/00 ,  C12N5/02 ,  C12N15/113

CPC分类号： C12Q1/6886 ,  C12N15/113 ,  C12N2310/113 ,  C12N2310/141 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/178

摘要： Disclosed herein are methods of diagnosing and treating a malignant adrenocortical tumor, including adrenocortical carcinoma. In some examples, methods of diagnosing a malignant adrenocortical tumor include detecting expression of at least one microRNA (miR) gene product, such as miR-100, miR-125b, miR-195, miR-483-3p, miR-483-5p and IGF2 mRNA in a sample obtained from the subject with an adrenocortical tumor and comparing expression of at least one of these miR gene products and IGF2 mRNA in the sample obtained from the subject to a control. Altered expression of at least one of the miR gene products and IGF2 mRNA, such as a decrease in miR-100, miR-125b or miR-195 or an increase in miR-483-3p, miR-483-5p, and an increase in IGF2 mRNA, in the sample obtained from the subject compared to the control indicates a malignant adrenocortical tumor.

摘要翻译： 本文公开了诊断和治疗恶性肾上腺皮质肿瘤包括肾上腺皮质癌的方法。 在一些实例中，诊断恶性肾上腺皮质肿瘤的方法包括检测至少一种微小RNA（miR）基因产物如miR-100，miR-125b，miR-195，miR-483-3p，miR-483-5p的表达 和IGF2 mRNA，并且将从受试者获得的样品中的至少一种这些miR基因产物和IGF2mRNA的表达与对照相比较。 miR基因产物和IGF2 mRNA中至少一种的改变表达，如miR-100，miR-125b或miR-195的减少或miR-483-3p，miR-483-5p的增加以及增加 在IGF2 mRNA中，与对照相比，获自受试者的样品显示恶性肾上腺皮质肿瘤。

公开(公告)号：US20120142753A1

公开(公告)日：2012-06-07

申请号：US12961298

申请日：2010-12-06

申请人： Electron Kebebew ,  Erin Patterson

发明人： Electron Kebebew ,  Erin Patterson

IPC分类号： A61K31/7088 ,  A61P35/00 ,  A61K31/713 ,  A61K31/7105 ,  C12Q1/68 ,  C40B30/00

CPC分类号： C12Q1/6886 ,  C12N15/113 ,  C12N2310/113 ,  C12N2310/141 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/178

摘要： Disclosed herein are methods of diagnosing and treating a malignant adrenocortical tumor, including adrenocortical carcinoma. In some examples, methods of diagnosing a malignant adrenocortical tumor include detecting expression of at least one microRNA (miR) gene product, such as miR-100, miR-125b, miR-195, miR-483-3p, miR-483-5p and IGF2 mRNA in a sample obtained from the subject with an adrenocortical tumor and comparing expression of at least one of these miR gene products and IGF2 mRNA in the sample obtained from the subject to a control. Altered expression of at least one of the miR gene products and IGF2 mRNA, such as a decrease in miR-100, miR-125b or miR-195 or an increase in miR-483-3p, miR-483-5p, and an increase in IGF2 mRNA, in the sample obtained from the subject compared to the control indicates a malignant adrenocortical tumor.

摘要翻译： 本文公开了诊断和治疗恶性肾上腺皮质肿瘤包括肾上腺皮质癌的方法。 在一些实例中，诊断恶性肾上腺皮质肿瘤的方法包括检测至少一种微小RNA（miR）基因产物如miR-100，miR-125b，miR-195，miR-483-3p，miR-483-5p的表达 和IGF2 mRNA，并且将从受试者获得的样品中的至少一种这些miR基因产物和IGF2mRNA的表达与对照相比较。 miR基因产物和IGF2 mRNA中至少一种的改变表达，如miR-100，miR-125b或miR-195的减少或miR-483-3p，miR-483-5p的增加以及增加 在IGF2 mRNA中，与对照相比，获自受试者的样品显示恶性肾上腺皮质肿瘤。