Method of removing nucleic acid contamination in amplification reactions
    3.
    发明授权
    Method of removing nucleic acid contamination in amplification reactions 有权
    消除扩增反应中核酸污染的方法

    公开(公告)号:US06541204B2

    公开(公告)日:2003-04-01

    申请号:US09485110

    申请日:2000-04-10

    IPC分类号: C12Q168

    摘要: The present invention provides a method of removing nucleic acid contamination in an amplification reaction which comprises use of a thermolabile DNase and a method of preventing or reducing false positive results due to carry-over in a nucleic acid amplification reaction, said method comprising using a thermolabile DNase to degrade carried-over non-target double-stranded DNA present in the amplification reaction mixture. A thermolabile DNase from the shrimp Pandalus borealis has been identified which is suitable for use in the methods of the invention.

    摘要翻译: 本发明提供一种除去扩增反应中的核酸污染的方法,该方法包括使用不耐热DNA酶,以及预防或减少由于核酸扩增反应中遗留导致的假阳性结果的方法,所述方法包括使用不耐热的 DNase降解扩增反应混合物中存在的携带的非靶双链DNA。 已经鉴定了适用于本发明方法的来自对虾Pandalus borealis的耐热不溶DNA酶。