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公开(公告)号：US5109121A
公开(公告)日：1992-04-28
申请号：US594459
申请日：1990-10-09
申请人： Galina Blum , Ren-der Yang , Eun K. Lee
发明人： Galina Blum , Ren-der Yang , Eun K. Lee
摘要： A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding Group IIA metal salts to the solution in amounts sufficient to selectively precipitate the high molecular weight protein contaminants is disclosed.The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.
摘要翻译： 公开了一种从含有高分子量污染蛋白质的蛋白质溶液中回收重组蛋白质的方法，通过直接向溶液中加入足以选择性沉淀高分子量蛋白质污染物的量的IIA族金属盐。 除去高分子量沉淀物，并进一步处理溶液以除去低分子量污染蛋白质和其它非蛋白质污染物。 随后回收重组蛋白并进一步加工以产生适合其预期用途的蛋白质组合物。
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公开(公告)号：US4988798A
公开(公告)日：1991-01-29
申请号：US468054
申请日：1990-01-22
申请人： Galina Blum , Ren-der Yang , Eun K. Lee
发明人： Galina Blum , Ren-der Yang , Eun K. Lee
摘要： A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding Group IIA metal salts to the solution in amounts sufficient to selectively precipitate the high molecular weight protein contaminants is disclosed.The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.
摘要翻译： 公开了一种从含有高分子量污染蛋白质的蛋白质溶液中回收重组蛋白质的方法，通过直接向溶液中加入足以选择性沉淀高分子量蛋白质污染物的量的IIA族金属盐。 除去高分子量沉淀物，并进一步处理溶液以除去低分子量污染蛋白质和其它非蛋白质污染物。 随后回收重组蛋白并进一步加工以产生适合其预期用途的蛋白质组合物。
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公开(公告)号：US06293026B1
公开(公告)日：2001-09-25
申请号：US09456576
申请日：1999-12-08
申请人： Eun K. Lee , Dong H. Kim , Hee T. Kim , Shin C. Kang , Young C. Kim , Sei K. Moon , Seong Y. Bae , Gyo T. Seo , Jong C. Lee , Choon K. Zhoh , Choong N. Cho , Jong S. Lee , Dae Y. Yang
发明人： Eun K. Lee , Dong H. Kim , Hee T. Kim , Shin C. Kang , Young C. Kim , Sei K. Moon , Seong Y. Bae , Gyo T. Seo , Jong C. Lee , Choon K. Zhoh , Choong N. Cho , Jong S. Lee , Dae Y. Yang
IPC分类号： A61B5107
摘要： A method of measuring the height of a foot arch precisely and easily using two essential heights of the foot arch is disclosed. In the method, a foot cast is primarily cast by pouring plaster into a foot mold. Thereafter, a first intersecting point and a second intersecting point are determined. The first intersecting point is determined by making a first line and a third line intersect. The second intersecting point is determined by making a second line and the third line intersect. The first line is a straight line extending from the center of the second toe to the center of the heel end. The second line is a straight line extending from the center of the big toe to a point spaced apart inwardly from the center of the heel end by ⅛ of the total distance of the heel end. The third line is a center line of the transverse arch of the foot. Finally, a first height and a second height are measured while the foot pattern is set in an upright position. The first height is defined between a flat base level and the first intersecting point, while the second height is defined between the base level and the second intersecting point.
摘要翻译： 公开了一种使用足弓的两个基本高度精确且容易地测量足弓的高度的方法。 在该方法中，脚浇铸主要是通过将石膏浇注到脚模中来铸造的。 此后，确定第一交点和第二交点。 通过使第一行和第三行相交来确定第一个相交点。 通过制作第二行和第三行相交来确定第二个相交点。 第一行是从第二脚趾的中心延伸到脚后跟中心的直线。 第二行是从大脚趾的中心延伸到从脚跟端的中心向内分开的距离为脚跟端的总距离的直线。 第三行是脚的横向拱的中心线。 最后，在将脚图案设置在直立位置时，测量第一高度和第二高度。 第一高度被限定在平坦的基准水平和第一个相交点之间，而第二个高度是在基准水平和第二个相交点之间定义的。
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