公开(公告)号：US10961542B2

公开(公告)日：2021-03-30

申请号：US14778190

申请日：2014-03-18

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA—EMBRAPA ,  FUNDAÇÃO UNIVERSIDADE DE BRASILIA—FUB—UNB

Inventor： Juliana Dantas de Almeida ,  Leila Maria Gomes Barros ,  Renata Henrique Santana ,  Ricardo Vilela Abdelnoor ,  Felipe Rodrigues da Silva

IPC: C12N15/82

Abstract: The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs that contain the promoter of the gene of the putative flavonol-sulfotransferase protein of soy plants for the production of transgenic plants, plant cells or protoplasts.

公开(公告)号：US20160272982A1

公开(公告)日：2016-09-22

申请号：US14778190

申请日：2014-03-18

Applicant: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA ,  FUNDAÇÃO UNIVERSIDADE DE BRASILIA - FUB - UNB

Inventor： Juliana Dantas de Almeida ,  Leila Maria Gomes Barros ,  Renata Henrique Santana ,  Ricardo Vilela Abdelnoor ,  Felipe Rodrigues da Silva

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8225 ,  C12N15/8227 ,  C12N15/8234 ,  C12N15/8241

Abstract: The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs that contain the promoter of the gene of the putative flavonol-sulfotransferase protein of soy plants for the production of transgenic plants, plant cells or protoplasts.

Abstract translation: 本发明涉及植物中基因表达的新启动子。 更具体地，本发明涉及从大豆植物中分离并可以引发和激活多核苷酸转录的多核苷酸的调节序列，以及这些调节序列用于修饰内源和/或异源多核苷酸的转录并产生多肽的调节序列。 本发明进一步描述了在大豆植物中含有假定的黄酮醇 - 磺基转移酶蛋白基因的启动子的DNA构建体，该启动子与异源和/或内源基因功能性连接。 此外，本发明涉及以表达盒，表达载体，重组载体和植物，植物细胞或转基因原生质体形式的这些构建体的用途。 本发明还描述了一种使用含有大豆植物推定的黄酮醇 - 磺基转移酶蛋白基因的启动子用于生产转基因植物，植物细胞或原生质体的构建体的方法。