公开(公告)号：US20070160994A1

公开(公告)日：2007-07-12

申请号：US10582613

申请日：2004-12-15

申请人： Annick Lim ,  Brigitte Marie-Christine Lemercier ,  Philippe Kourilsky ,  Francois Huetz

发明人： Annick Lim ,  Brigitte Marie-Christine Lemercier ,  Philippe Kourilsky ,  Francois Huetz

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6881 ,  C12Q2600/156

摘要： The present invention provides a process for determining the quantitative and qualitative profile of the repertoire of a given type of an immunoglobulin (Ig) heavy chain expressed by a lymphocyte B population present in a tissue sample, and kits and uses thereof. It also provides a set of VII forward primers associated with a CII reverse primer, which are respectively capable of specifically hybridizing in stringent conditions with the nucleic acids encoding the variable segments of Ig heavy chains and with the constant segment of a given type of Ig heavy chain, such as preferably IgM, IgG, IgE or IgA heavy chain. Methods for the in vitrodiagnosis of a condition associated with an abnormal expression of the repertoire of a given type of Ig heavy chain, and for the in vitro follow-up of a treatment of such a condition, are also comprised herein.

摘要翻译： 本发明提供了确定由组织样品中存在的淋巴细胞B群体表达的给定类型的免疫球蛋白（Ig）重链的所有组成成分的定量和定性分析的方法及其试剂盒和用途。 它还提供了与CII反向引物相关的一组VII正向引物，其能够在严格条件下与编码Ig重链的可变区的核酸以及给定类型的Ig重量的恒定区特异性杂交 链，例如优选IgM，IgG，IgE或IgA重链。 与特定类型的Ig重链的所有组成成分异常表达相关的病症的体外诊断方法以及对这种病症的治疗的体外随访也包括在本文中。

公开(公告)号：US20080166718A1

公开(公告)日：2008-07-10

申请号：US11822280

申请日：2007-07-03

申请人： Annick Lim ,  Brigitte Lemercier ,  Philippe Kourilsky ,  Francois Huetz

发明人： Annick Lim ,  Brigitte Lemercier ,  Philippe Kourilsky ,  Francois Huetz

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6876 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/56 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present invention provides a process for determining the quantitative and qualitative profile of the repertoire of a given type of an immunoglobulin (Ig) heavy chain expressed by a B lymphocyte population present in a tissue sample, and kits and uses thereof. It also provides a set of VH forward primers associated with a CH reverse primer, which are respectively capable of specifically hybridizing in stringent conditions with the nucleic acids encoding the variable segments of Ig heavy chains and with the constant segment of a given type of Ig heavy chain, such as preferably an IgM, IgG, IgE or IgA heavy chain. Methods for the in vitro diagnosis of a condition associated with an abnormal expression of the repertoire of a given type of Ig heavy chain, and for the in vitro follow-up of a treatment of such a condition, are also included herein.

摘要翻译： 本发明提供了确定由组织样品中存在的B淋巴细胞群表达的给定类型的免疫球蛋白（Ig）重链的所有组成成分的定量和定性分析的方法及其试剂盒和用途。 它还提供了一组与CH反向引物相关的VH正向引物，其分别能够在严格条件下与编码Ig重链的可变区的核酸以及给定类型的Ig重量的恒定区特异性杂交 链，例如优选IgM，IgG，IgE或IgA重链。 用于体外诊断与给定类型的Ig重链的所有组成成分异常表达有关的病症的方法以及对这种病症的治疗的体外随访。