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公开(公告)号：US08026075B2
公开(公告)日：2011-09-27
申请号：US12977874
申请日：2010-12-23
申请人： Fumitomo Odawara
发明人： Fumitomo Odawara
摘要： Disclosed is a sugar and/or a sugar alcohol as a substance for suppressing dephosphorylation reaction of a phosphorylated coenzyme. Also disclosed is a method for stabilizing a phosphorylated coenzyme which is characterized by having at least a substance for suppressing dephosphorylation reaction of the phosphorylated coenzyme coexist with the phosphorylated coenzyme.
摘要翻译： 公开了作为抑制磷酸化辅酶的脱磷酸化反应的物质的糖和/或糖醇。 还公开了一种稳定磷酸化辅酶的方法，其特征在于至少具有抑制磷酸化辅酶的去磷酸化反应与磷酸化辅酶共存的物质。
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公开(公告)号：US20080318256A1
公开(公告)日：2008-12-25
申请号：US11576048
申请日：2005-10-05
申请人： Fumitomo Odawara
发明人： Fumitomo Odawara
IPC分类号： G01N33/543 , C07F9/6512
摘要： Disclosed is a sugar and/or a sugar alcohol as a substance for suppressing dephosphorylation reaction of a phosphorylated coenzyme. Also disclosed is a method for stabilizing a phosphorylated coenzyme which is characterized by having at least a substance for suppressing dephosphorylation reaction of the phosphorylated coenzyme coexist with the phosphorylated coenzyme.
摘要翻译： 公开了作为抑制磷酸化辅酶的去磷酸化反应的物质的糖和/或糖醇。 还公开了一种稳定磷酸化辅酶的方法，其特征在于至少具有抑制磷酸化辅酶的去磷酸化反应与磷酸化辅酶共存的物质。
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公开(公告)号：US5935834A
公开(公告)日：1999-08-10
申请号：US836380
申请日：1997-05-07
申请人： Fumitomo Odawara
发明人： Fumitomo Odawara
CPC分类号： C12N9/1276
摘要： Disclosed is a reverse transcriptase composition having improved storage stability, comprising a reverse transcriptase, an effective stabilizing amount of at least one organic stabilizing reagent selected from trehalose and a nucleic acid containing a transcriptional initiation site recognizable by the enzyme, and an effective stabilizing amount of a metal salt capable of producing bivalent positive ions in an aqueous solution of the metal salt. Also disclosed is a method for improving storage stability of a reverse transcriptase, which comprises adding the above-mentioned organic stabilizing reagent and metal salt to a reverse transcriptase. The composition of the present invention can be stably stored for a prolonged period of time at a temperature up to at least 4.degree. C. Further, by virtue of a relatively high temperature usable for stable storage, the viscosity of the composition can be advantageously maintained at a low level, so that it becomes possible to accurately dispense the composition by a quantity corresponding to a desired enzyme activity, thereby achieving high reproducibility in experiments using the reverse transcriptase. Therefore, in the determination of a virus, in which a reverse transcriptase activity is used as an index, the composition of the present invention can be advantageously used as a standard substance for determining the amount of virus.
摘要翻译： PCT No.PCT / JP95 / 02304 Sec。 371日期1997年5月7日 102（e）日期1997年5月7日PCT提交1995年11月10日PCT公布。 出版物WO96 / 15235 日期1996年5月23日公开是具有改善的储存稳定性的逆转录酶组合物，其包含逆转录酶，有效稳定量的至少一种选自海藻糖的有机稳定剂和含有可被酶识别的转录起始位点的核酸， 有效稳定金属盐水溶液中能够产生二价正离子的金属盐。 还公开了一种用于提高逆转录酶的储存稳定性的方法，其包括将上述有机稳定剂和金属盐加入到逆转录酶中。 本发明的组合物可以在高达至少4℃的温度下稳定地保存较长时间。此外，由于可用于稳定储存的相对较高的温度，可以有利地保持组合物的粘度 在低水平上，使得可以以对应于所需酶活性的量精确地分配组合物，从而在使用逆转录酶的实验中实现高再现性。 因此，在使用逆转录酶活性作为指标的病毒的测定中，本发明的组合物可以有利地用作用于确定病毒量的标准物质。
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公开(公告)号：US20110159564A1
公开(公告)日：2011-06-30
申请号：US12977874
申请日：2010-12-23
申请人： Fumitomo ODAWARA
发明人： Fumitomo ODAWARA
IPC分类号： C12N9/96
摘要： Disclosed is a sugar and/or a sugar alcohol as a substance for suppressing dephosphorylation reaction of a phosphorylated coenzyme. Also disclosed is a method for stabilizing a phosphorylated coenzyme which is characterized by having at least a substance for suppressing dephosphorylation reaction of the phosphorylated coenzyme coexist with the phosphorylated coenzyme.
摘要翻译： 公开了作为抑制磷酸化辅酶的脱磷酸化反应的物质的糖和/或糖醇。 还公开了一种稳定磷酸化辅酶的方法，其特征在于至少具有抑制磷酸化辅酶的去磷酸化反应与磷酸化辅酶共存的物质。
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5.发明授权
公开(公告)号：US5989819A
公开(公告)日：1999-11-23
申请号：US952282
申请日：1997-11-18
申请人： Fumitomo Odawara
发明人： Fumitomo Odawara
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/569 , G01N33/573
CPC分类号： G01N33/573 , C12Q1/6804 , C12Q1/703 , G01N33/56988 , G01N2333/9128
摘要： Disclosed is a method for quantitatively determining an antibody, contained in a biological sample, having the ability to inhibit the activity of a reverse transcriptase derived from an HIV, comprising: reacting a hybridization product, immobilized on a solid phase, of a primer consisting essentially of oligodeoxythymine nucleotide and an RNA template consisting essentially of adenine ribopolynucleotide with a reverse transcriptase derived from HIV, to thereby bind the reverse transcriptase to the hybridization product; conducting a synthesis of a DNA complementary to the RNA template from a deoxymononucleotide triphosphate in the presence of the reverse transcriptase activity-inhibiting antibody; and measuring the amount of the synthesized DNA and quantitatively determining the above-mentioned antibody, based on the measured amount of the synthesized DNA. The method of the present invention can be performed with ease and with high reproducibility in classifying and quantitatively determining the antibodies against the epitopes of a viral reverse transcriptase. By the method of the present invention, not only the virus having mutated during a latent period thereof, and the properties which the virulis exhibits as a result of the mutation, but also a virus having acquired drug resistance as a result of the administration of the drugs to the host, can be studied by quantitatively determining the antibodies. Further, it has also become possible to accurately and promptly determine the changes in the infected host, such as the change in the ability to produce antibodies, which occur in accordance with the progression of illness.
摘要翻译： PCT No.PCT / JP96 / 01407 Sec。 371日期：1997年11月18日 102（e）1997年11月18日PCT PCT 1996年5月24日PCT公布。 公开号WO96 / 37781 日期1996年11月28日公开是一种用于定量测定生物样品中包含的具有抑制源自HIV的逆转录酶活性的能力的抗体的方法，包括：将固定在固相上的杂交产物， 基本上由寡脱氧胸腺嘧啶核苷酸组成的引物和基本上由腺嘌呤核糖核苷酸与源自HIV的逆转录酶组成的RNA模板，从而将逆转录酶与杂交产物结合; 在逆转录酶活性抑制性抗体的存在下，从脱氧单核苷酸三磷酸进行与RNA模板互补的DNA的合成; 并测定合成DNA的量，并根据合成DNA的测定量定量测定上述抗体。 本发明的方法可以容易地进行，并且对于针对病毒逆转录酶的表位的抗体的分类和定量测定具有高的重复性。 通过本发明的方法，不仅在其潜伏期期间突变的病毒，以及毒力因突变而显示的性质，而且是由于施用有效性而获得耐药性的病毒 可以通过定量测定抗体来研究宿主的药物。 此外，还可以准确和及时地确定受感染宿主的变化，例如根据疾病进展发生的产生抗体的能力的变化。
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公开(公告)号：US07879567B2
公开(公告)日：2011-02-01
申请号：US11576048
申请日：2005-10-05
申请人： Fumitomo Odawara
发明人： Fumitomo Odawara
摘要： Disclosed is a sugar and/or a sugar alcohol as a substance for suppressing dephosphorylation reaction of a phosphorylated coenzyme. Also disclosed is a method for stabilizing a phosphorylated coenzyme which is characterized by having at least a substance for suppressing dephosphorylation reaction of the phosphorylated coenzyme coexist with the phosphorylated coenzyme.
摘要翻译： 公开了作为抑制磷酸化辅酶的去磷酸化反应的物质的糖和/或糖醇。 还公开了一种稳定磷酸化辅酶的方法，其特征在于至少具有抑制磷酸化辅酶的去磷酸化反应与磷酸化辅酶共存的物质。
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