公开(公告)号：US10975366B2

公开(公告)日：2021-04-13

申请号：US16476878

申请日：2017-10-24

申请人： GUANGDONG VTR BIO-TECH CO., LTD

发明人： Yangyuan Li ,  Jianrong Wang ,  Ling Yang ,  Man Qu ,  Lizhi Chen ,  Jiale Huang

IPC分类号： C12N9/54 ,  C12N9/44

摘要： The present invention relates to the field of genetic engineering, particularly to a method for efficiently expressing pullulanase in Bacillus subtilis and recombinant Bacillus subtilis. said method includes steps of constructing modified Bacillus subtilis strain with deletion of alkaline protease gene and neutral protease gene, constructing expression vector including an optimized combination of promoter and signal peptide and pullulanase gene, and transforming said modified Bacillus subtilis strain with by said expression vector. A series of combinations of promoter and signal peptide are optimized to obtain the combination for efficiently expressing pullulanase, provide an industrial application basis.