公开(公告)号：US20230178183A1

公开(公告)日：2023-06-08

申请号：US17922340

申请日：2021-04-30

申请人： GeneMind Biosciences Co., Ltd.

发明人： Jicai Fan ,  Huan Jin ,  Fang Chen ,  Lei Sun

IPC分类号： G16B30/10 ,  C12Q1/6869 ,  C12N15/10 ,  G06N7/01

CPC分类号： G16B30/10 ,  C12Q1/6869 ,  C12N15/1068 ,  G06N7/01

摘要： An effective sequencing method, the method comprising: (1) performing first sequencing on a sequencing template on a chip surface, so as to facilitate obtaining first sequencing data by means of first newly generated sequencing strands being formed, the sequencing template being connected onto the chip surface by means of a sequencing adapter; (2) performing first blocking treatment on 3′ ends of at least a portion of the first newly generated sequencing strands; and (3) performing second sequencing on the sequencing template, so as to facilitate obtaining second sequencing data by means of second newly generated sequencing strands being formed.

公开(公告)号：US20240279367A1

公开(公告)日：2024-08-22

申请号：US18020915

申请日：2021-08-10

申请人： GeneMind Biosciences Co., Ltd.

发明人： Diewen FENG ,  Zhifeng LIN ,  Jicai FAN ,  Lei LIU ,  Fang CHEN ,  Qi WANG

IPC分类号： C08F8/08 ,  C12Q1/6837

CPC分类号： C08F8/08 ,  C12Q1/6837

摘要： The present application relates to an amino-modified chip, preparation method thereof and use thereof. The amino-modified chip comprises: a substrate and a high polymer, wherein the substrate is modified with epoxy groups; and the high polymer is attached to the substrate via the epoxy groups, at least one structural unit of the high polymer contains an amino, and the amino is a primary amino or a secondary amino.

公开(公告)号：US20240035084A1

公开(公告)日：2024-02-01

申请号：US18037056

申请日：2021-11-11

申请人： GeneMind Biosciences Co., Ltd.

发明人： Qi WANG ,  Lei SUN ,  Diewen FENG ,  Jinhong GAO ,  Zhifeng LIN ,  Lei LIU ,  Fang CHEN

IPC分类号： C12Q1/6874 ,  C09D143/04

CPC分类号： C12Q1/6874 ,  C09D143/04

摘要： The present application relates to a chip, a preparation method therefor, and an application thereof. The chip of embodiments of the present application comprises: a substrate having a surface grafted with an amino group, the amino group being a primary amino group or a secondary amino group; and a first compound linked to the surface via the amino group, the first compound comprising the following structure, i.e., formula (1) or formula (2), wherein R1 and R2 are each independently selected from —OH or C1-C5 alkoxy, and n is 1 or 2. The chip can be used for a nucleic acid test, such as single-molecule sequencing and next-generation sequencing.