公开(公告)号：US20140363846A1

公开(公告)日：2014-12-11

申请号：US14241101

申请日：2012-08-24

申请人： Jason B. Edwards ,  Glenn Munkvold

发明人： Jason B. Edwards ,  Glenn Munkvold

IPC分类号： C12N9/24 ,  C12P19/14

CPC分类号： C12N9/2402 ,  C12N1/14 ,  C12N9/2437 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/137 ,  D06P5/158

摘要： The present invention provides a fermentation process for producing a cellulase enzyme mixture comprising the steps of (a) providing a fungal cell of the genus Myceliophthora or a taxonomically equivalent genus; (b) culturing the fungal cell in a submerged liquid batch culture in which the carbon source comprises about 0% of cellulase-inducing carbon source; (c) culturing the fungal cell from the batch culture from step (b) in a submerged liquid fed-batch and/or continuous culture; and (d) providing the culture of step (c) with a feed solution containing a about 100 wt % non-inducing carbon source (such as glucose, dextrose, sucrose, molasses, fructose, glycerol, xylose, or a combination thereof), wherein the feed solution is provided at a rate that maintains the concentration of non-inducing carbon source in the culture below that which would otherwise repress production of the cellulase enzyme mixture, so as to produce a culture filtrate containing at least 10 g protein/L.

摘要翻译： 本发明提供了一种用于生产纤维素酶混合物的发酵方法，其包括以下步骤：（a）提供霉菌属真菌细胞或分类学上等同的属; （b）在浸没的液体分批培养物中培养真菌细胞，其中碳源包含约0％的纤维素酶诱导碳源; （c）在浸没液体补料分批和/或连续培养中从步骤（b）的批次培养物中培养真菌细胞; 和（d）提供步骤（c）的培养物，其中含有约100重量％的非诱导性碳源（如葡萄糖，右旋糖，蔗糖，糖蜜，果糖，甘油，木糖或其组合）的进料溶液， 其中所述进料溶液以使培养物中的非诱导性碳源的浓度保持在低于否则会抑制纤维素酶混合物的产生的浓度的速率下提供，以产生含有至少10g蛋白质/ L的培养物滤液 。